Fluorescence and FRET in Membranes
Fluorescence methodologies are based on emission of light from a substance in an electronically excited singlet state. Förster Resonance Energy Transfer (FRET) denotes a photophysical process upon which the excitation energy of a molecule, the donor, is transferred to another, the acceptor, without emission of light from the former or collision between the two species.
The molecular level organization and structure/function relationships in membrane systems pose challenging problems that require a variety of experimental approaches for their study. Among these, and due to its sensitivity and versatility, fluorescence came to the fore. The timescale of fluorescence (sub-ns resolution) is adequate to follow the kinetics of both fast (fluorophore rotation, conformational changes) and relatively slow (translocation, hindered diffusion) processes. Its intrinsic sensitivity is almost unrivalled and...
- Loura LMS, Prieto M (2013) Lateral membrane heterogeneity probed by FRET spectroscopy and microscopy. In: Mély Y, Duportail G (eds) Fluorescent methods to study biological membranes. Springer, Berlin, pp 71–113Google Scholar