Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Single Fluorophore Photobleaching

  • Qinsi Zheng
  • Avik Kumar Pati
  • Scott C. Blanchard
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_482-1

Definition

Photobleaching describes any process that leads to the permanent loss of fluorescence from light-emitting fluorescent species, including organic dye molecules, semiconductor particles such as quantum dots, and fluorescent proteins upon exposure to photons or light. As such processes terminate the flux of photons emanating from a fluorophore, photobleaching serves as a key limiting factor in applications relying on light emission from a fluorophore. Such applications include medical imaging technologies, diagnostics (Diaspro et al. 2006), dye-sensitized solar cells (Grätzel 2003), and light-emitting diodes (Xu et al. 2016).

Basic Characteristics

Photobleaching is caused by photochemical damage of the fluorophore species. Such processes are enhanced by prolonged and intense illumination and are related directly to the probability of photoinduced reactions with solvent and intramolecular reactions of the fluorophore itself that can occur during cycles of photon absorption and...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  • Qinsi Zheng
    • 1
  • Avik Kumar Pati
    • 2
  • Scott C. Blanchard
    • 1
    • 2
  1. 1.Tri-Institutional Program in Chemical BiologyWeill Cornell MedicineNew YorkUSA
  2. 2.Department of Physiology and BiophysicsWeill Cornell MedicineNew YorkUSA

Section editors and affiliations

  • Nils G. Walter
    • 1
  1. 1.Department of ChemistryThe University of MichiganAnn ArborUSA