Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Total Correlation Spectroscopy (TOCSY) in NMR Protein Structure Determination

  • Seth ChitayatEmail author
  • Steven P. Smith
  • David N. Langelaan
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_351-1


Due to extensive chemical shift overlap and degeneracy, protein side-chain resonance assignment is tedious and difficult for many structural biologists. This technical challenge creates a major bottleneck in generating solution-based protein structures using nuclear magnetic resonance (NMR) spectroscopy, since resonance chemical shifts are required to assign 1H-1H nuclear Overhauser effect (NOE) distance restraints for subsequent structure calculation. However, access to high-field NMR spectrometers as well as major improvements in the manner in which NMR data is collected, processed, and analyzed has helped overcome this challenge. Specifically, total correlation spectroscopy (TOCSY), also referred to as homonuclear Hartmann-Hahn (HOHAHA), has been an important development in the field. In the TOCSY experiment, correlations are observed between coupled nuclei of the same isotope, allowing for straightforward assignment of side-chain 1H, 13C, and 15N resonances. This entry...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  • Seth Chitayat
    • 1
    Email author
  • Steven P. Smith
    • 1
  • David N. Langelaan
    • 2
  1. 1.Department of Biomedical and Molecular SciencesQueen’s UniversityKingstonCanada
  2. 2.Department of Biochemistry & Molecular BiologyDalhousie UniversityHalifaxCanada

Section editors and affiliations

  • Mitsu Ikura

There are no affiliations available