Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Nucleic Acid NMR: Introduction

  • Masato Katahira
  • Tsukasa Mashima
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_302-1

Synonyms

DNA

Definition

Nucleic acid NMR is the study of the DNA/RNA structure in solution with the NMR method.

Introduction

NMR is a powerful tool to study the DNA/RNA structure in solution. It should be reminded that there are many examples in which the structure of a certain DNA/RNA in solution is quite different from that in crystal. This entry describes how the DNA/RNA structure can be determined by the NMR method. In addition to the established ways of the analysis, recent progresses and tips are also documented.

Firstly, three different ways of the DNA/RNA preparation are described. Secondly, the method of the resonance assignment, that is, the crucial step for the NMR analysis, is explained. Thirdly, the way to identify the mode of the base pair, that is, the key information to construct the structure, is described. Fourthly, the methods to derive various structural restraints are explained. Fifthly, the way to determine the structure by calculation by means of structural...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Kyoto University, Institute of Advanced EnergyKyotoJapan

Section editors and affiliations

  • Mitsu Ikura

There are no affiliations available