Nucleic Acid NMR: Introduction
Nucleic acid NMR is the study of the DNA/RNA structure in solution with the NMR method.
NMR is a powerful tool to study the DNA/RNA structure in solution. It should be reminded that there are many examples in which the structure of a certain DNA/RNA in solution is quite different from that in crystal. This entry describes how the DNA/RNA structure can be determined by the NMR method. In addition to the established ways of the analysis, recent progresses and tips are also documented.
Firstly, three different ways of the DNA/RNA preparation are described. Secondly, the method of the resonance assignment, that is, the crucial step for the NMR analysis, is explained. Thirdly, the way to identify the mode of the base pair, that is, the key information to construct the structure, is described. Fourthly, the methods to derive various structural restraints are explained. Fifthly, the way to determine the structure by calculation by means of structural...