Software Tools for Biological Structural Analysis Using Small-Angle X-Ray Solution Scattering
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Small-angle scattering of X-rays (SAXS) is a powerful method for the low-resolution structural analysis of biological macromolecules in solution. The technique provides three-dimensional low-resolution structures, using ab initio and rigid body modelling, and allows a researcher to assess the oligomeric state of proteins and protein complexes. In addition, SAXS is a powerful tool for structure validation and the quantitative analysis of flexible systems and is highly complementary to the high-resolution methods of X-ray crystallography (see “Macromolecular Crystallography”) and NMR (see “NMR”). At present, SAXS analysis methods allow one to perform automated and rapid characterization of protein solutions in terms of low-resolution models, quaternary structures, and oligomeric composition. A brief overview of software tools available for the analysis of proteins in solution using SAXS is presented here.
Primary Data Reduction and Processing
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