Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Mass Spectrometry: Mapping Large Stable Protein Complexes

  • Tara PukalaEmail author
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_187-1



Large, stable protein complexes are assemblies of two or more polypeptide chains, linked by specific noncovalent protein–protein interactions, which are stable on a biologically relevant timescale.

Mapping protein complexes is the process of determining noncovalent binding interactions and quaternary structure of protein subunits in a multiprotein assembly.


The large majority of cellular processes are carried out by a sophisticated network of protein machinery. Knowledge of these molecular interactions is vital to understanding and controlling the biological processes of the cell. Therefore, closely connected to the study of protein function is the analysis of protein structure and identification of binding partners. In many contexts, this is described as “mapping protein complexes,” which currently forms a challenging yet rich area...

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© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Discipline of ChemistryUniversity of AdelaideAdelaideAustralia