Definition
UapA is a member of the nucleobase ascorbate transporter (NAT) family, also known as the nucleobase-cation-symport-2 (NCS-2) family, designated A.2.40 in the Transporter Classification Database (TCDB) (Saier et al. 2006, 2009, 2016). See also Kourkoulou et al., Nucleobase-Ascorbate-Transporter (NAT) Family this volume. UapA is a H+-dependent symporter responsible for uptake of the purines, xanthine and uric acid. The wild-type protein contains 574 residues (Diallinas and Scazzocchio 1989). A modified construct containing a single point mutation (G411V) and lacking the N-terminal 11 amino acids termed UapAG411VΔ1−11 (Leung et al. 2013) was used for recombinant expression, isolation, crystallization, and structure determination. This protein binds xanthine (Leung et al. 2013) and effectively traffics to the membrane but is transport inactive (Koukaki et al. 2005). The purified UapAG411VΔ1−11 protein is sufficiently stable for crystallization (Alguel et al. 2016) and analysis...
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Alguel, Y. et al. (2018). Structural Aspects of UapA the H+-Xanthine/Uric Acid Transporter from Aspergillus nidulans. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_10089-1
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DOI: https://doi.org/10.1007/978-3-642-35943-9_10089-1
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