Encyclopedia of Pain

2013 Edition
| Editors: Gerald F. Gebhart, Robert F. Schmidt

Hot Plate Test (Assay)

Reference work entry
DOI: https://doi.org/10.1007/978-3-642-28753-4_200958


The hot plate test involves placement of a rat or mouse on a heated surface (usually 50 °C) and measuring the latency (in seconds) to licking of a hindpaw or jumping. The hot plate test, which employs a fixed temperature, has largely been supplanted by what is termed the “Hargreaves Test” in which a rat or mouse is placed on a glass surface and the animal’s hindpaw is heated from below by a radiant heat source. Latency from stimulus to withdrawal of the paw is measured in this test.


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