Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Trichuris suis Artificial Larval Hatching

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4469-1
The larvae of Trichuris suis – whipworm of pigs – can be used to stimulate the immune system of humans and thus block for up to 3 months of autoimmune reactions which lead to symptoms such as Morbus Crohn or ulcerative colitis. In cases of treatment, about 2,000 larvae-containing eggs have to be swallowed within some milliliter of water. In order to control, whether a sufficient number of larvae hatched and remained motile inside the intestine, a so-called hatch test must be done from time to time with stored eggs. By the way the larvae of T. suis do not reach maturity in humans, but die within 3 months after hatch. The process needs the following steps:
  1. 1.

    Incubation of the eggs in a 13 % hypochlorite solution at 40 °C and 5 % CO2 for 2–5 h

     
  2. 2.

    Washing and centrifugation (1,000 rpm) within a Trichuris-hatching medium (see below)

     
  3. 3.

    Treatment with ultrasonic waves for 1 min

     
  4. 4.

    Transfer of treated eggs into plastic wells with hatching medium at 37–40 °C

     
  5. 5.
    Microscopical...

Keywords

Immune System Ulcerative Colitis Fetal Calf Serum Calf Serum Ultrasonic Wave 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Reading

  1. Bager P et al (2011) Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial. PLoS ONE 6(8):e22346CrossRefPubMedPubMedCentralGoogle Scholar
  2. Garud S, Peppercorn MA (2009) Ulcerative colitis: current treatment strategies and future prospects. Therap Adv Gastroenterol 2:99–108CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany