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Natural Products pp 2813-2851 | Cite as

Metabolic Engineering of Isoprenoid Biosynthesis

  • Anne Pribat
  • Lisa Boureau
  • Anne Mortain-Bertrand
  • Linda S. Bert
  • Dominique Rolin
  • Emeline Teyssier
  • Philippe Gallusci
Reference work entry
First Online:

Abstract

Isoprenoids constitute one of the largest families of natural compounds. They play essential functions in plant growth and development and furnish compounds of high interest for humans. Here, we present the current knowledge on isoprenoid metabolism before describing the strategies that have been used for isoprenoid metabolic engineering. We discuss the advantages and drawbacks of using microorganisms and plants as cell platform for the production of isoprenoids of interest.

Keywords

Carotenoid Essential oil Isoprenoid Metabolic engineering Methylerythritol phosphate Mevalonate Terpene synthase Terpenoid Transgenic plants Volatile terpene 

Abbreviations

ADS

Amorpha 4,11-diene synthase

CRY2

Cryptochrome 2

DET1

De-etiolated 1

DMAPP

Dimethylallyl diphosphate

DXP

1-deoxy-d-xylulose 5-phosphate

DXR

1-deoxy-d-xylulose 5-phosphate

FPP

Farnesyl diphosphate

FPS

Farnesyl diphosphate synthase

GGPP

Geranyl geranyl diphosphate

GGPS

Geranyl geranyl diphosphate synthase

GPP

Geranyl diphosphate

GPS

Geranyl diphosphate synthase

HMG

3-hydroxyl-3-methylglutaryl

HMGR

3-hydroxyl-3-methylglutaryl reductase

IDS

Isoprenyl diphosphate synthase

IPP

Isopentenyl diphosphate

IS

Isoprene synthase

MEP

Methyl-d-erythritol 4-phosphate

MGT

Multigene transfer

MVA

Acetate/mevalonate

NES1

Nerolidol synthase

NPP

Neryl diphosphate

PSY

Phytoene synthase

PTS

Prenyltransferase

SqS

Squalene synthase

TPS

Terpene synthase

VOCS

Volatile organic compounds.

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Notes

Acknowledgments

We wish to thank Dr Sophie Colombié for critical reading of this manuscript. Dr Anne Pribat was in receipt of a grant from the French National Research Agency (ANR) in the frame of the “Polyterp” project.

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Anne Pribat
    • 1
  • Lisa Boureau
    • 1
  • Anne Mortain-Bertrand
    • 2
  • Linda S. Bert
    • 1
  • Dominique Rolin
    • 2
  • Emeline Teyssier
    • 2
  • Philippe Gallusci
    • 1
  1. 1.Université de Bordeaux 1, UMR 1332 de Biologie du Fruit et PathologieVillenave d’OrnonFrance
  2. 2.Université de Bordeaux 1, INRA, UMR 1332 de Biologie du Fruit et PathologieVillenave d’OrnonFrance

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