High-Speed Atomic Force Microscopy (AFM)
High-speed AFM (HS-AFM) is a type of atomic force microscopy (AFM) that, unlike conventional AFM, can take an image very quickly and with relatively low imaging force, therefore allowing for visualizing the dynamic behavior of biomolecules.
AFM can take high-resolution images of objects in various environments. In particular, its in-liquid imaging capability is useful for biological research. However, the imaging rate of conventional AFM is too low to capture dynamic processes of biomolecules (DNA, proteins). HS-AFM has been developed to overcome this problem by optimizing all devices contained in the instrument for fast scanning and by newly developing fast and precise control techniques (Ando et al. 2001, 2008). The imaging rate of the current HS-AFM has reached 15–25 frames per second (fps) for a scan range of ∼240×240 nm2with 100 scan lines. By further reducing the scan range, the imaging rate can exceed 50 fps....
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