Methods for Studying Methanogens and Methanogenesis in Marine Sediments
Despite the global importance of microbial methane production, knowledge of the methanogens involved and related anaerobic methane-oxidizing Euryarchaea (ANME) is quite limited. This includes the controls on methane production and consumption, which determines the atmospheric concentration of methane, which is an important greenhouse gas involved in climate change. This situation, in part, reflects the difficulty of culturing and studying these fastidious anaerobes. Here, we describe a range of cultivation and cultivation independent approaches that we have used to study methanogens, methanogenesis, and related processes in marine sediments, which contain globally significant methane concentrations.
KeywordsMarine Sediment Butyl Rubber Stopper mcrA Gene Anaerobic Methane Oxidizer Methanogen Enrichment
We are grateful for grants from the UK Natural Environment Research Council and the European Union to conduct much of this research.
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