Chimeric Antigen Receptor on T Cells
Synonyms
CAR
Definition
A chimeric antigen receptor (CAR) consists of an extracellular antigen-binding exodomain, typically derived from a single-chain antibody fragment (scFv) of a monoclonal antibody (mAb), a spacer (such as an antibody Fc region), a transmembrane region, and one or more intracellular signaling endodomains, which can be genetically introduced into hematopoietic cells, such as T cells, to redirect specificity for a desired cell-surface antigen.
Characteristics
Background on Manipulating T-Cell Responses to Cancer
Adoptive transfer of tumor-specific T cells in mouse models can function as potent anti-cancer biological agents leading to elimination of established malignancies. Continued advances in tumor immunology support the premise and promise for adoptive immunotherapyas a treatment for human malignancies. Yet, infusion of tumor-specific T cells has only been partially successful in clinical oncology trials. Indeed, most of these trials demonstrate the safety and...
References
- 1.Eshhar Z, Waks T, Bendavid A et al. (2001) Functional expression of chimeric receptor genes in human T cells. J Immunol Methods 248(1–2):67–76PubMedCrossRefGoogle Scholar
- 2.Rossig C, Brenner MK (2003) Chimeric T-cell receptors for the targeting of cancer cells. Acta Haematol 110(203):154–159PubMedGoogle Scholar
- 3.Cooper L, Topp MS, Serrano LM et al. (2003) T-cell clones can be rendered specific for CD19: toward the selective augmentation of the graft-versus-B-lineage leukemia effect. Blood 101:1637–1644PubMedCrossRefGoogle Scholar
- 4.Morgan RA, Dudley ME, Wunderlich JR et al. (2006) Cancer regression in patients after transfer of genetically engineered lymphocytes. Science 314:126–129PubMedCrossRefGoogle Scholar
- 5.Park JR, Digiusto DL, Slovak M et al. (2007) Adoptive transfer of chimeric antigen receptor re-directed cytolytic T lymphocyte clones in patients with neuroblastoma. Mol Ther 15(4):825–833PubMedGoogle Scholar