Cellulose-Based Nanosupports for Enzyme Immobilization
Integration of biocatalysts and nanoscale materials offer multiple advantages over micro-scaled heterogeneous biocatalysts. Apart from providing reusability and sustainability of the enzyme, the use of nanosupports is aimed at increasing the surface area available for biocatalyst immobilization and improving the yields in bioconversions through better biocatalyst mobility and less diffusional problems. Among many nanomaterials for enzyme immobilization, cellulose stands out as biocompatible, biodegradable, and environmentally-friendly regarding its biological source. In this chapter, we discuss the steady advancement in utilizing different nanostructured cellulosic materials for enzyme immobilization. We address the use of hybrid materials that include cellulose and improve the properties of the heterogeneous biocatalyst. The methodologies for functionalization and integration of enzymes on nanocellulose hydrogels are discussed including covalent linkage through chemical modification, entrapment, and cross-linking. We consider its applications to biomedicine, food industry, and environmental science with a special emphasis on the impact of the enzymatic properties caused after immobilization on cellulosic supports.
KeywordsEnzyme immobilization Cellulose Nanosupports Biocatalysis Nanobiotechnology
The authors acknowledge ANII, PEDECIBA, and Universidad ORT Uruguay for providing financial support.
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