Somatic Cell Nuclear Transfer
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Somatic cell nuclear transfer (SCNT) is a technique which transfers the nucleus of a donor somatic cell to the cytoplasm of an oocyte with its own nucleus removed. The somatic nucleus is reprogrammed in the oocyte, in which it contains the entire genomic information of the somatic cell and progressively develops into the blastocyst. Successive development of the blastocyst with proper stimuli can result in generation of cloned animals in reproductive cloning or embryonic stem cells (ESC) for cloning specific tissues and cell types.
Somatic cell nuclear transfer is a classical technique in biotechnology for reprogramming the nucleus of a somatic cell (Singh et al. 2019). The cell can resume pluripotency and further differentiate from the embryonic state into specific tissues and cell types that inherited the original genotype in the donor nucleus. With the embryotic cell being grown in a surrogate mother, a...