In Vitro Delayed-Type Sensitivity Testing
Although patch testing is regarded to be the most standardized and reliable method for diagnosis of delayed-type hypersensitivity to contact allergens, its substitution by in vitro techniques would be advantageous for several reasons.
The lymphocyte transformation test (LTT) measures the allergen-specific proliferation or activation of T cells in vitro and has been explored as potential alternative for patch testing for decades. Beside numerous attempts to increase its sensitivity and specificity, the major drawback of a cell culture-based method is the poor solubility of many contact sensitizers and the need for chemical modification of prohaptens.
The LTT is used with regularity only for diagnosis of hypersensitivity to beryllium and adverse reactions to drugs. Its usefulness in occupational dermatology is limited and restricted to special situations as a supplement for patch testing with large panels of well-established sensitizers.
KeywordsPatch testing Lymphocyte transformation test Delayed-type hypersensitivity Beryllium Drug hypersensitivity In vitro testing MELISA Nickel Mercury T cells ELISpot Cytokines Cell proliferation Sensitivity Specificity
Lymphocyte transformation test
Peripheral blood mononuclear cells
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