Flotillin-1 was originally identified in 1997, when Bickel et al. analyzed caveolin-rich membrane fractions from mouse lung and isolated a 45 kDa protein that was microsequenced. Degenerate primers were designed from the peptide sequences and used for PCR of mouse lung cDNA. The PCR-product was then used as a probe for screening of a 3T3-L1 adipocyte cDNA library. The characterized sequence showed similarity to the ESA protein. An antipeptide antibody identified a 47 kDa protein on Western blots. Because this protein was detected in floating, caveolin-rich, Triton X-100-insoluble membrane fractions, it was termed “flotillin-1.” The similar ESA protein (47% identity) was renamed as “flotillin-2.” Also in 1997, Schulte et al. identified and characterized two proteins that were induced in regenerating fish retinal ganglion cell axons after injury and thus were named “reggie-1” and “reggie-2.” In 1998, Lang et al. cloned the rat reggie...
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