The adhesion and degranulation promoting adapter protein (ADAP) is a large protein which is alternatively spliced to produce a 120 or a 130 kDa isoform and is expressed in T cells, proB cells (but not mature B cells), and myeloid cells. ADAP was first cloned in 1997 by two independent labs that were working to elucidate signaling pathways induced by engagement of the T cell receptor. In early studies, the kinase, Fyn-T, was found to be an important mediator of mature, peripheral T cell activation. In order to identify potential substrates of Fyn-T, da Silva et al. used GST–FYN-SH2 domain fusion proteins to immunoprecipitate a 120/130 kDa phosphorylated protein from a TCR/antiCD3 stimulated T cell line. An antibody developed against this protein was used to screen a Jurkat cell line cDNA library and led to the cloning of ADAP. In the same year, Musci et al. used a chimeric...
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