Historical Background
The adhesion and degranulation promoting adapter protein (ADAP) is a large protein which is alternatively spliced to produce a 120 or a 130 kDa isoform and is expressed in T cells, proB cells (but not mature B cells), and myeloid cells. ADAP was first cloned in 1997 by two independent labs that were working to elucidate signaling pathways induced by engagement of the T cell receptor. In early studies, the kinase, Fyn-T, was found to be an important mediator of mature, peripheral T cell activation. In order to identify potential substrates of Fyn-T, da Silva et al. used GST–FYN-SH2 domain fusion proteins to immunoprecipitate a 120/130 kDa phosphorylated protein from a TCR/antiCD3 stimulated T cell line. An antibody developed against this protein was used to screen a Jurkat cell line cDNA library and led to the cloning of ADAP. In the same year, Musci et al. used a chimeric...
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Kasirer-Friede, A. (2018). ADAP. In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, Cham. https://doi.org/10.1007/978-3-319-67199-4_577
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DOI: https://doi.org/10.1007/978-3-319-67199-4_577
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