For many years now, several studies in steroid-producing systems have led to the postulation of an induced stimulatory protein originally designated as the “labile protein factor,” since after stimulation with ACTH in the presence of a protein synthesis inhibitor as cycloheximide (CHX), the rate of steroidogenesis rapidly returned to its unstimulated level (Haynes et al. 1959; Garren et al. 1965; Schulster et al. 1970).
Using two-dimension gel electrophoresis, one of the first papers in the matter identified a protein of 28,000 Da, present as different isoforms in hormone-stimulated adrenal cells but not in quiescent cells. Furthermore, 35S-methionine pulse-chase experiments showed that this protein is not produced from a preexisting one, even if protein synthesis is not inhibited (Krueger and Orme-Johnson 1983).
After that work, Pon, Hartigan, and Orme-Johnson showed in 1986 that...
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