Encyclopedia of Signaling Molecules

2018 Edition
| Editors: Sangdun Choi


  • Jennifer Rha
  • Stephanie K. Jones
  • Anita H. CorbettEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-3-319-67199-4_101743


Historical Background

From budding yeast to humans.

ZC3H14 (zinc finger CysCysCysHis-type containing 14), which is also termed MSUT-2, is a ubiquitously expressed polyadenosine RNA-binding protein that has been characterized in a number of model organisms. The best studied orthologue of ZC3H14 is the Nab2 (nuclear polyadenylated RNA-binding 2) protein in budding yeast (S. cerevisiae), which was originally identified in a biochemical screen for poly(A) RNA-binding proteins (Anderson et al. 1993). To identify these proteins, budding yeast cells were UV irradiated to crosslink protein to RNA. Polyadenylated RNAs were then selectively purified using oligo(dT)-sepharose beads, and the polyadenylated RNA-protein complexes isolated...

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Jennifer Rha
    • 1
  • Stephanie K. Jones
    • 1
  • Anita H. Corbett
    • 2
    • 3
    Email author
  1. 1.Emory UniversityAtlantaUSA
  2. 2.Department of BiologyEmory UniversityAtlantaUSA
  3. 3.Department of BiochemistryEmory University School of MedicineAtlantaUSA