From budding yeast to humans.
ZC3H14 (zinc finger CysCysCysHis-type containing 14), which is also termed MSUT-2, is a ubiquitously expressed polyadenosine RNA-binding protein that has been characterized in a number of model organisms. The best studied orthologue of ZC3H14 is the Nab2 (nuclear polyadenylated RNA-binding 2) protein in budding yeast (S. cerevisiae), which was originally identified in a biochemical screen for poly(A) RNA-binding proteins (Anderson et al. 1993). To identify these proteins, budding yeast cells were UV irradiated to crosslink protein to RNA. Polyadenylated RNAs were then selectively purified using oligo(dT)-sepharose beads, and the polyadenylated RNA-protein complexes isolated...
- Soucek S, Zeng Y, Bellur DL, Bergkessel M, Morris KJ, Deng Q, et al. The evolutionarily-conserved polyadenosine RNA binding protein, Nab2, cooperates with splicing machinery to regulate the fate of pre-mRNA. Mol. Cell. Biol. 2016;36:21(18):2697–2714.Google Scholar