RNA processing is a critical step in gene expression. RNA-binding proteins are involved in every aspect of RNA processing including splicing, polyadenylation, nuclear export, translation, localization, and, eventually, RNA turnover. Polyadenylate-binding nuclear protein 1 (PABPN1) is an RNA-binding protein that is predominantly localized to the nucleus where its canonical function is to regulate polyadenosine [poly(A)] tail addition by first stimulating poly(A) polymerase (PAP) activity and then limiting poly(A) tail length (Wahle 1991, Kuhn et al. 2009). PABPN1 is also involved in RNA export from the nucleus, the pioneer round of translation, and targeting some specific RNAs for decay (Banerjee et al. 2013). Although PABPN1 was originally cloned and characterized as an RNA recognition motif (RRM)-containing RNA-binding protein, interest in the protein greatly increased when mutations that modestly expand an N-terminal...
- Kuhn U, Gundel M, Knoth A, Kerwitz Y, Rudel S, Wahle E. Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor. J Biol Chem. 2009;284(34):22803–14.PubMedPubMedCentralCrossRefGoogle Scholar