NTCP (Sodium Taurocholate Cotransporting Polypeptide)
A sodium-dependent transport system for conjugated bile salts was extensively characterized in early studies using perfused rat liver and isolated rat and human basolateral membranes. The factor responsible for the activity of the system was first identified by Dr. Meier’s group in 1990. In their seminal study, they injected rat liver poly (A)+ RNA into Xenopus laevis oocytes and observed the functional expression of sodium-dependent taurocholate uptake (Hagenbuch et al. 1990). Meier and coworkers size-fractionated and enriched the active mRNA, and showed that the 1.5∼3 Kb subfraction supported bile salt uptake. Following expression cloning of a rat cDNA library, they succeeded in cloning rat Ntcp, which consists of 1738 nucleotides that encode an open reading frame and whose expression produces a 362 aa polypeptide (Hagenbuch et al. 1991). NTCP was subsequently cloned...
- Hou, X., Mao F., Jing Z., Liu X., Li W. Inactivation of Ntcp causes gallbladder diseases in mice. International meeting on molecular biology of hepatitis B viruses; 2015, Bad Nauheim, Germany, p. 0–177.Google Scholar