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In Vitro Allergy Testing

  • Brian P. PeppersEmail author
  • Robert Hostoffer
  • Theodore Sher
Living reference work entry

Abstract

In vitro allergy testing has been utilized, in various methods, for almost 100 years. This chapter will primarily focus on clinically available laboratory allergy testing. Details on how the specific allergy tests are conducted and performed will be reviewed. Current clinical correlation of specific allergy test results is discussed. Practical utilization and limitations on in vitro IgE and IgG testing will be explored. Brief introduction to experimental in vitro allergy testing for research is covered.

Keywords

Laboratory allergy testing In vitro allergy testing IgE allergy testing 

References

  1. Ballmer-Weber BK, Lidholm J, Fernández-Rivas M, Seneviratne S, Hanschmann KM, Vogel L, Bures P, Fritsche P, Summers C, Knulst AC, Le TM. IgE recognition patterns in peanut allergy are age dependent: perspectives of the EuroPrevall study. Allergy. 2015;70(4):391–407.CrossRefGoogle Scholar
  2. Bernstein IL, Li JT, Bernstein DI, Hamilton R, Spector SL, Tan R, Sicherer S, Golden DB, Khan DA, Nicklas RA, Portnoy JM. Allergy diagnostic testing: an updated practice parameter. Ann Allergy Asthma Immunol. 2008;100(3):S1–48.CrossRefGoogle Scholar
  3. Borres MP, Maruyama N, Sato S, Ebisawa M. Recent advances in component resolved diagnosis in food allergy. Allergol Int. 2016;65(4):378–87.CrossRefGoogle Scholar
  4. Branum AM, Lukacs SL. Food allergy among children in the United States. Pediatrics. 2009;124(6):1549–55.CrossRefGoogle Scholar
  5. Busse W, Corren J, Lanier BQ, McAlary M, Fowler-Taylor A, Della Cioppa G, van As A, Gupta N. Omalizumab, anti-IgE recombinant humanized monoclonal antibody, for the treatment of severe allergic asthma. J Allergy Clin Immunol. 2001;108(2):184–90.CrossRefGoogle Scholar
  6. College of American Pathologists website. www.cap.org. Retrieved on 16 Aug 2018.
  7. Cox L, Nelson H, Lockey R, Calabria C, Chacko T, Finegold I, Nelson M, Weber R, Bernstein DI, Blessing-Moore J, Khan DA. Allergen immunotherapy: a practice parameter third update. J Allergy Clin Immunol. 2011;127(1):S1–55.CrossRefGoogle Scholar
  8. Deak PE, Vrabel MR, Kiziltepe T, Bilgicer B. Determination of crucial immunogenic epitopes in major peanut allergy protein, Ara h2, via novel nanoallergen platform. Sci Rep. 2017;7:3981.CrossRefGoogle Scholar
  9. Du Toit G, Roberts G, Sayre PH, Plaut M, Bahnson HT, Mitchell H, Radulovic S, Chan S, Fox A, Turcanu V, Lack G. Identifying infants at high risk of peanut allergy: the Learning Early About Peanut Allergy (LEAP) screening study. J Allergy Clin Immunol. 2013;131(1):135–43.CrossRefGoogle Scholar
  10. Glaumann S, Nilsson C, Johansson SG, Asarnoj A, Wickman M, Borres MP, Nopp A. Evaluation of basophil allergen threshold sensitivity (CD-sens) to peanut and Ara h 8 in children IgE-sensitized to Ara h 8. Clin Mol Allergy. 2015;13(1):5.CrossRefGoogle Scholar
  11. Golden DB, Kagey-Sobotka A, Norman PS, Hamilton RG, Lichtenstein LM. Insect sting allergy with negative venom skin test responses. J Allergy Clin Immunol. 2001;107(5):897–901.CrossRefGoogle Scholar
  12. Golden DB, Demain J, Freeman T, Graft D, Tankersley M, Tracy J, Blessing-Moore J, Bernstein D, Dinakar C, Greenhawt M, Khan D. Stinging insect hypersensitivity. Ann Allergy Asthma Immunol. 2017;118(1):28–54.CrossRefGoogle Scholar
  13. Hamilton RG. Microarray technology applied to human allergic disease. Microarrays. 2017;6(1):3.CrossRefGoogle Scholar
  14. Haneda Y, Kando N, Yasui M, Kobayashi T, Maeda T, Hino A, Hasegawa S, Ichiyama T, Ito K. Ovomucoids IgE is a better marker than egg white–specific IgE to diagnose boiled egg allergy. J Allergy Clin Immunol. 2012;129(6):1681–2.CrossRefGoogle Scholar
  15. Lundberg GD, Iverson C, Radulescu G. Now read this: the SI units are here. Am J Dis Child. 1986;140(6):513–23.PubMedGoogle Scholar
  16. McGowan EC, Saini S. Update on the performance and application of basophil activation tests. Curr Allergy Asthma Rep. 2013;13(1):101–9.CrossRefGoogle Scholar
  17. Meurant G. Immunoassay: a practical guide. Singapore: Pan Stanford Publishing Pte. Ltd; 2012.Google Scholar
  18. Muraro A, Lemanske RF, Castells M, Torres MJ, Khan D, Simon HU, Bindslev-Jensen C, Burks W, Poulsen LK, Sampson HA, Worm M. Precision medicine in allergic disease–food allergy, drug allergy, and anaphylaxis-PRACTALL document of the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma & Immunology. Allergy. 2017;72(7):1006–21.CrossRefGoogle Scholar
  19. O’Kennedy R, Murphy C. Immunoassays development, applications and future trends. Singapore: Pan Stanford; 2017.CrossRefGoogle Scholar
  20. Omalizumab Full prescribing information. https://www.gene.com/download/pdf/xolair_prescribing.pdf. Retrieved on 16 Aug 2018.
  21. Patrick LF, Linda AB, Lisa AF, Alsabeh R, Regan SF, Jeffrey DG, Thomas SH, Karabakhtsian RG, Patti AL, Marolt MJ, Steven SS. Principles of analytic validation of immunohistochemical assays. Arch Pathol Lab Med. 2014;138(11):1432–43.CrossRefGoogle Scholar
  22. Patterson K, Strek ME. Allergic bronchopulmonary aspergillosis. Proc Am Thorac Soc. 2010;7(3):237–44.CrossRefGoogle Scholar
  23. Peddecord KM, Hammond HC. Clinical laboratory regulation under the Clinical Laboratory Improvement Amendments of 1988: can it be done? Clin Chem. 1990;36(12):2027–35.PubMedGoogle Scholar
  24. Reddy A, Greenberger PA. Allergic bronchopulmonary aspergillosis. J Allergy Clin Immunol Pract. 2017;5(3):866–7.CrossRefGoogle Scholar
  25. Rondón C, Campo P, Togias A, Fokkens WJ, Durham SR, Powe DG, Mullol J, Blanca M. Local allergic rhinitis: concept, pathophysiology, and management. J Allergy Clin Immunol. 2012;129(6):1460–7.CrossRefGoogle Scholar
  26. Ruëff F, Wenderoth A, Przybilla B. Patients still reacting to a sting challenge while receiving conventional Hymenoptera venom immunotherapy are protected by increased venom doses. J Allergy Clin Immunol. 2001;108(6):1027–32.CrossRefGoogle Scholar
  27. Sampson HA. Utility of food-specific IgE concentrations in predicting symptomatic food allergy. J Allergy Clin Immunol. 2001;107(5):891–6.CrossRefGoogle Scholar
  28. Sampson HA, Aceves S, Bock SA, James J, Jones S, Lang D, Nadeau K, Nowak-Wegrzyn A, Oppenheimer J, Perry TT, Randolph C. Food allergy: a practice parameter update – 2014. J Allergy Clin Immunol. 2014;134(5):1016–25.CrossRefGoogle Scholar
  29. Santos AF, Brough HA. Making the most of in vitro tests to diagnose food allergy. J Allergy Clin Immunol Pract. 2017;5(2):237–48.CrossRefGoogle Scholar
  30. Sicherer SH, Morrow EH, Sampson HA. Dose-response in double-blind, placebo-controlled oral food challenges in children with atopic dermatitis. J Allergy Clin Immunol. 2000;105(3):582–6.CrossRefGoogle Scholar
  31. Thorpe SJ, Heath A, Fox B, Patel D, Egner W. The 3rd International Standard for serum IgE: international collaborative study to evaluate a candidate preparation. Clin Chem Lab Med. 2014;52(9):1283–9.CrossRefGoogle Scholar
  32. Vereda A, van Hage M, Ahlstedt S, Ibañez MD, Cuesta-Herranz J, van Odijk J, Wickman M, Sampson HA. Peanut allergy: clinical and immunologic differences among patients from 3 different geographic regions. J Allergy Clin Immunol. 2011;127(3):603–7.CrossRefGoogle Scholar
  33. Wells JV, Buckley RH, Schanfield MS, Fudenberg HH. Anaphylactic reactions to plasma infusions in patients with hypogammaglobulinemia and anti-IgA antibodies. Clin Immunol Immunopathol. 1977;8(2):265–71.CrossRefGoogle Scholar
  34. Yalow RS, Berson SA. Assay of plasma insulin in human subjects by immunological methods. Nature. 1959;184(4699):1648–9.CrossRefGoogle Scholar
  35. Zeng Q, Dong SY, Wu LX, Li H, Sun ZJ, Li JB, Jiang HX, Chen ZH, Wang QB, Chen WW. Variable food-specific IgG antibody levels in healthy and symptomatic Chinese adults. PLoS One. 2013;8(1):e53612.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Brian P. Peppers
    • 3
    Email author
  • Robert Hostoffer
    • 1
    • 2
    • 3
  • Theodore Sher
    • 1
    • 2
    • 3
  1. 1.Department of Adult PulmonaryUniversity Hospitals Cleveland Medical CenterClevelandUSA
  2. 2.Allergy and Immunology Associates, Inc.Mayfield HeightsUSA
  3. 3.Division of Allergy and ImmunologyWVU Medicine Children’sMorgantownUSA

Section editors and affiliations

  • Timothy Craig
    • 1
  1. 1.Department of Medicine and PediatricsPenn State College of MedicineHersheyUSA

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