Cis–Trans Isomerase of Unsaturated Fatty Acids: An Immediate Bacterial Adaptive Mechanism to Cope with Emerging Membrane Perturbation Caused by Toxic Hydrocarbons

Reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)

Abstract

A rather efficient solvent adaptation mechanism enabling several gram-negative bacteria to tolerate and grow in the presence of membrane-disturbing compounds is the isomerization of cis- to trans-unsaturated membrane fatty acids. The degree of isomerization obviously depends on the toxicity and the concentration of membrane-affecting agents. Synthesis of trans-fatty acids comes about by direct isomerization of the respective cis-configuration of the double bond without shifting the position. The purpose of the conversion of the cis-configuration to trans is apparently the rapid adaptation of the membrane fluidity to rising temperature or the presence of toxic organic hydrocarbons.

The cis–trans-isomerase (Cti) is a constitutively expressed periplasmic enzyme that – to exert its action – necessitates neither ATP nor other cofactors, and consistently, is independent of de novo synthesis of lipids. A heme-binding site typical of cytochrome c-type proteins is present in the predicted Cti polypeptide indicating a reaction mechanism that renounces temporary saturation of the double bond. Due to its direct correlation with toxicity, cis–trans-isomerization is a potential biomarker for recording solvent stress or changes of other environmental conditions.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Hermann J. Heipieper
    • 1
  • J. Fischer
    • 3
  • F. Meinhardt
    • 2
  1. 1.Department Environmental BiotechnologyHelmholtz Centre for Environmental Research – UFZLeipzigGermany
  2. 2.Institut für Molekulare Mikrobiologie und BiotechnologieWestfälische Wilhelms-Universität MünsterMünsterGermany
  3. 3.Department of Environmental BiotechnologyHelmholtz Centre for Environmental ResearchLeipzigGermany

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