Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability
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Polymers are considered as a versatile class of materials due to their enormous contributions that helped the mankind to reach the comfort level that we enjoy in today’s society. On the other hand, their extensive use creates various threats to the environment in terms of pollution to the atmosphere, various health hazards to humans and other living species, and moreover weakening of nonrenewable natural assets. The last few decades witnessed a paradigm shift toward development and commercialization of polymers from bio-based renewable resources that can suit very well for all those applications achieved by their petroleum-based counterparts. The present chapter analyzes in detail about the various life stages, starting from the sources, for a very important and widely commercialized biopolymer, polyhydroxybutyrate (PHB). Recent developments in blends and composites of PHB with other polymers and fillers are discussed in terms of structure-property relationships and applications. Their contribution toward the preservation of the ecosystem and the sustainability is also described.
KeywordsPHB Biomaterials Composites Biopolymer Sustainability
Biopolymers and Ecosystem
There is a serious concern related to the damages caused by waste plastics to the ecosystem. Littering of used/waste plastics leads to a serious threat to the ecosystem as a whole. The reason for this concern is the growing demand and hence the growth in the production of plastics all over the world. According to a recent data published by Plastics Europe , the world plastic production in the year 2015 reached almost 322 metric tons. with an annual growth rate of 8.4%. Interestingly, this figure does not include polyethylene terephthalate (PET), polyamides (PA), and polyacrylic fibers. A serious consequence of this rapid growth in production and consumption of plastics all over the world is the generation of large volumes of used plastic wastes. Another study reports that almost 60% of all those plastic materials ever produced all over the world are discarded and they are accumulated in landfills or end up as litter in the environment . A gargantuan amount of these waste plastics are accumulated in the marine environment and cause a serious threat to the marine ecosystem [3, 4]. A scientific study reports that around 5.25 trillion plastic particles weighing 268,940 tons are currently floating in the sea . If the trend continues like this, then the prediction is that by 2050, the sea will contain more plastic waste than fish.
First of all, they reduce the dependency on nonrenewable fossil fuels for their synthesis.
Biopolymers are compostable, and hence there is less chance of environmental pollution compared with their petroleum- or fossil fuel-originated counterparts.
Biopolymers are considered as carbon neutral and sustainable as they are originated from renewable sources.
Biopolymers reduce carbon emissions and reduce the carbon dioxide levels in the atmosphere.
PHB as a Biopolymer
PHB-based blends and their applications 
Drug delivery system
Production of disposable goods like food trays, cups, etc.
Biomedical devices and packaging goods
Commercially available PHA/PHB grades in the market 
Grades suitable for
Mitsubishi gas chemicals, Japan
Injection molding, extrusion
PHB industrial, Brazil
Injection molding, extrusion, thermoforming
Injection molding, cast film, thermoforming
CheilJedang, South Korea
Injection molding, extrusion
Tianan biol. Mat., China
Injection molding, extrusion,
P&G chemicals, Japan
Injection molding, extrusion, thermoforming
Biomed Biotechnol. Germany
Injection molding, extrusion
Sources of PHB
Poly-3-hydroxybutyrate (PHB) is a linear polyester, which was first discovered in bacteria by Lemoigne in 1925 [21, 22]. The most important factor in production of PHB is that their biological production from non-replenishing sources by bacterial systems occurs in both gram-positive and gram-negative bacteria under the nutrient imbalance condition . More than 20 bacterial species such as Bacillus megaterium, Methylobacterium rhodesianum, Alcaligenes eutrophus, M. extorquens, P. putida, Sphaerotilus natans, Escherichia coli, etc. are known as effective producers of PHB .
The chemolithotrophic bacteria like Ralstonia eutropha, Cupriavidus metallidurans, and Alcaligenes latus are capable of producing PHB from simple carbon sources. Among gram-positive bacteria, Bacillus spp., B. subtilis, Bacillus thuringiensis, and B. cereus and, among gram-negative bacteria, Pseudomonas spp. are notable for the production of PHB. A. eutropha is a gram-negative bacteria, capable of accumulating PHB, from simple carbon substrates such as glucose, palm oil, molasses, and cereal grains . The microbial cells store PHB in the form of about 0.5 μm diameter granules, in the cytoplasmic fluid of the producing organism in well-defined subcellular complex sections called carbonosomes . Bacterial species gather intracellular polyhydroxybutarates granules as energy and carbon reserves within their cells. Media play a vital function for the production of PHB. Molasses, corn steep liquor, wheat bran, starch, etc. are some of the cheap sources of media. The other important factors are carbon, nitrogen sources, pH, temperature, pressure, media flow rate, oxygen supply, etc. .
Bacterial PHB production belongs to two main groups; the first can be induced by deficiency of essential nutrients such as nitrogen, sulfate, phosphate, potassium, magnesium, etc., and the second group synthesizes PHB by the normal logarithmic growth stage. In the process, microorganisms are unable to synthesize amino acid but produce PHB and accumulate it as discrete granules in the presence of excess carbon. Glucose can be restored by starch, molasses, whey, activated sludge, etc. as the carbon source to enhance PHB yield. Utilization of cheap substrates will eventually decrease the production cost. Among different microorganisms, Alcaligenes eutrophus, a gram-negative bacteria, employs many advantages as it gathers PHB at up to 80% of its dry weight, is safe to handle in large quantities, grows efficiently on glucose as a carbon source, and is able to produce the industrially significant PHBV copolymer when propionic acid is added to the glucose feedstocks. PHBV copolymers with 30 mol% of hydroxyvalerate are formed, the hydroxyvalerate fraction being controlled by varying the ratio of glucose to propionic acid in the feedstock [14, 25, 27].
Life Stages of PHB
During polymerization, a skin is formed around the granule to separate the polymers from the aqueous cytosol. PHB granules are surrounded by a thick proteinaceous layer known as phasing which decides the size of the PHB granule and shields the granule in vivo . After fermentation, bacterial cells containing polymer granules are separated from the medium by centrifugation. The polymers are easily recovered from the cells through solvent extraction. The intracellular PHB granules show amorphous characteristics, but the obtained extracellular PHB granules after solvent extraction show a semicrystalline configuration with an ordered molecular arrangement . PHB granules accumulated in bacterial cells as the amorphous state are easily accessible by PHB depolymerases. PHB depolymerases use the presence of water, which is an integral part of the system. Freezing and storage of polymer granules at low temperature or by treatment with solvents during extraction activate irreversible conversion of PHB to the crystalline form . Electron and X-ray diffraction data on single PHB crystals confirm an orthorhombic crystal system and indicate a fiber repeat of 5.9 Å . Organic solvents like acetone, chloroform, methylene chloride, etc. are involved in the solvent extraction methods to recover the intracellular PHB. The requirement of organic solvents makes the process economically and environmentally disagreeable. But, for medical products, the solvent extraction is a good technique due to obtaining PHBs with a high purity. There are many potential alternative methods to the solvent extraction. Aqueous enzymatic procedures, treatments with ammonia, and digestion with sodium hypochlorites and surfactant are some of the alternative methods of the critical solvent extraction proposed for the recovery of PHBs. A new farming method allows the spontaneous release of up to 95% PHB per cell dry weight from Escherichia coli [14, 32]. The cost of PHB depends on the cost of carbon sources, equipment used, and downstream processing techniques. The scale-up of PHB production builds up recombinant strains capable of metabolizing different carbon sources. E. coli is a generally established recombinant system in terms of PHB production. E. coli has a delicate cell wall which makes it a suitable host for the PHB production. A promising strategy involves genetic engineering of microorganisms for the more efficient production of PHB . Biosynthesis of PHB consists of three structural genes phbC, phbA, and phbB: the phbC codes involved in the polymerization of 3-hydroxybutyryl-CoA monomer to poly-3-hydroxybutyryl-CoA, phbA codes involved in the condensation of two acetyl-CoA molecules to form acetoacetyl-CoA, and phbB codes for the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA [12, 33].
Blends and Composites of PHB
PHB is an environmentally friendly polymer and has a lot of applications in packaging and biomedical fields. However, the phenomenon called embrittlement limits the use of the polymer over a period of time due to the enhanced brittleness of the polymer . In order to curb this issue, blending with other polymer is recommended. Several studies related to PHB blends are reported recently, and a short account of them is described in the following sections.
High-molecular-weight components in polymer blends influence the morphology and thereby the properties. Sharma et al. investigated the effect of ultrahigh-molecular-weight PHB in PHB on the morphology by a semi-continuous shear flow technique . They found that the shishes formed by the said technique is stable for 5–10 min and on blending with different propotions of PHB stable fibers were formed. When the shear was severe (10–20), the formation of fibers became oriented, and stable morphology was obtained.
Hong et al. investigated the effect of blending a small amount of carboxyl-terminated butadiene acrylonitrile rubber (CTBN) and biocompatible polyvinylpyrrolidone (PVP) with PHB on the crystallization and thermal degradation properties . The blends were analyzed with respect to melting behavior, crystallinity, size of the crystals formed, the rate of crystallization, thermal stability, and degradation kinetics. It was found that the addition of both PVP and CTBN in small amounts greatly affected the degradation reaction. Other properties also showed significant improvements, for example, both thermal stability and crystallization rate were enhanced. 1 wt% of PVP was seen as the optimum and showed the maximum enhancement in crystallization and degradation behaviors.
Kumagai and Doi tried to blend PHB with poly(e-caprolactone) (PCL), poly(1,4-butylene adipate) (PBA), and poly(vinyl acetate) (PVAc) in different proportions . The binary blends were investigated based on miscibility, morphology, and biodegradability. Out of the three systems, only PHB/PAc blends were miscible, and the other two were immiscible as evidenced by glass transition temperatures from DSC. Both the immiscible blends have phase-separated structure which was confirmed from the mechanical properties and SEM images. The enzymatic degradation of the blends was conducted at 37°C at a pH of 7.4, and it was confirmed that the blend component with PHB has a greater influence on the degradation properties. As the weight percentage of the component increased especially for PVAc, the weight loss decreased gradually, and this behavior was later correlated with the morphology of the blends.
Barham and Organ studied the mechanical properties of PHB blends with hydroxyvalerate prepared by extrusion process . They confirmed that at certain temperature and compositions, these blends form phase separation. The yield and fracture properties were correlated with crystallization aspects of the blends. Also, the effects of ageing at room temperature on the mechanical properties were investigated, and it was found that the properties get deteriorated over the course of time. Solution casting method using chloroform was used to prepare PHB with polyvinyl acetate and polyvinyl acetate-co-vinyl alcohol to get improved materials with physical properties . The miscibility and biological applications of the prepared materials were investigated.
Recent developments in blends of PHB
PHB, low-density polyethene (LDPE), and polyamide (PA)
Solvent casting from chloroform-ethanol solvent combination and by melt/solid extrusion with a single-screw extruder
Water transport, morphology, FTIR
A simple method to prepare biodegradable blends proposed based on the results
Poly(lactic acid) (PLA) and PHB were blended and plasticized with a natural terpene D-limonene (LIM)
Mixing in HAAKE PolyLab mixer at 180 °C followed by molding in hot press at 180 °C
DSC, TSC, FTIR, SEM, biodegradation, wettability, statistical analysis
Improved water-resistant and barrier properties, biodegradable in compost, miscible blends
PLA, PHB, and thermoplastic starch
Casting 1% (w/v) in dimethylene chloride (DMC)
TGA, degradation studies, and SEM
Considerable improvement (98%) in biodegradation
PHB and starch
Mixing in HAAKE PolyLab mixer at 175 °C followed by molding in hot press at 180 °C
FTIR, DSC, DMA, rheology, tensile tests, optical microscopy
Hydrogen bonding between PHB and starch improved thermal stability, melt viscosity, and degradation
PHB and acrylonitrile-g-(ethylene-co-propylene-co-diene)-g-styrene (AES)
Batch mixing and twin-screw extrusion
DSC, DMA, impact resistance, SEM
Multifold increase in impact resistance or 30 wt% of AES
Poly[(R)3-hydroxybutyrate] (PHB), poly(L-lactic acid) (PLLA) with poly(PHB/PLLA urethane) as a compatibilizer
Solution casting in chloroform followed by drying under vacuum
Morphology, mechanical properties, SAXS, DSC, WAXS, GPC
Compatibilizer improved miscibility and properties to use the blends as biomedical devices
PHB, PLA, nanocellulose
Disintegration by composting conditions, viscosity, mechanical properties, statistical analysis
Nanocellulose decreased the disintegration of the blends, thereby suggested for food packaging applications
PHB, poly(3-hydroxyoctanoate) (PHO)
Solution casting using chloroform to get artificial granules
Density gradient analysis, NMR, DSC
Two phases were detected and amorphous elastomeric nature of polymers retained
Nanofibers from blends of PVA and PHB
Electrospinning of blend solutions
FTIR, DSC, SEM, tem
Blends were miscible, and hydrolytic degradation of PHB decreased with PLA addition. Cell culture experiments proposed to use the materials as scaffolds
PLA, PHB blends two different plasticizers, poly(ethylene glycol) (PEG) and acetyl-tri-n-butyl citrate (ATBC)
Melt blending and compression molding
Degradation under composting conditions, TGA, SEM
PHB as nucleating agent slowed down the disintegration, while plasticizers speeded it up
Maize starch, PHB
Solution blending in chloroform
Color, opacity, FTIR, SEM, DSC
Heterogeneous dispersion of starch granules seen. Potential to use as packaging materials
Composites of PHB with other heterogeneous materials such as metal powders, nanotubes, fibers, fillers, etc. were prepared to improve the properties. Natural and synthetic fibers were used as potential reinforcements. Rajan et al. investigated the effect of chitosan on the mechanical and thermal properties of PHB up to 40 wt% of filler loading . The composites were prepared by micro-compounding at a temperature of 175 °C, and the pelletized resultant materials were injection molded at appropriate conditions. Young’s modulus of PHB showed significant improvement with respect to the chitosan loading as well as glass transition temperature. Thermal stability of PHB did not improve much.
In order to improve the thermal stability near the melting point of PHB, two different nanoclays were incorporated by D’Amico et al. . Two types of nanocomposites with montmorillonite Cloisite® Na+ and organomodified Cloisite®C15A were prepared by solution casting in chloroform. Mechanical, thermal, and crystallization characteristics were analyzed with appropriate techniques. The organomodified clay improved mechanical and crystallization properties compared to the former one. The more hydrophobic clay showed better dispersion due to chain mobility and catalytic activity.
The centrifugal spinning method was used to prepare PHB pectin composite fibers by Foster and Tighe to examine the in vitro hydrolytic degradation . This method is more sophisticated than the conventional spinning technique. The hydrolytic degradation was conducted at 70 °C and at a pH of 10.6 with varying loadings of pectin in PHB. The degradation profile was greatly influenced by the pectin types used, and the materials were suggested for potential applications in wound dressing.
Macedo et al. did an interesting work with coir dust as the filler for PHB . Coir dust is a biowaste generated during the processing of coir, and it contains short fiber and powder. The samples were prepared using an internal mixer and followed by hot pressing at suitable temperature and pressure. The loading of coir dust was varied from 0 to 30 wt%. From the different analytical techniques, it was confirmed that the 10 wt% loading has the optimum properties. Even though the matrix polymer is hydrophobic in nature and the filler is hydrophilic, a certain degree of interaction between them is visible at low loading due to surface features in the filler.
In order to utilize PHB as a hard tissue replacement owing to its biocompatibility, a composite with particulate hydroxyapatite (HA) was prepared . The composites with varying weight percentages of HA up to 30 were prepared via compounding, milling, and molding techniques. In vitro tests in simulated body fluid (SBF) and other conventional tests were done to examine the suitability of the composites. The resultant composites showed high in vitro bioactivity in accordance with the volume percentage of HA. Initially, HA forms a layer on the composite surface as evidenced from SEM images, and prolonged immersion in SBF degrades it. Thus, the prepared composites are recommended for use as biodegradable materials for hard tissue replacement and regeneration.
Krishnaprasad et al. used bamboo microfibrils extracted from bamboo as reinforcements for PHB for different loadings . The composites were prepared by compounding, pelletization, and microinjection molding at a temperature of 175 °C. The microfibrils showed irregular surface morphology as evidenced by SEM, thereby improving the mechanical properties due to the better adhesion between the polymer and fibrils. The optimum concentration of microfibrils was 10%. The optimum loading was again confirmed from impact strength analysis .
Cabedo et al. analyzed the degradation of PHB during processing with different nanoclays . Processing styles such as internal mixing, extrusion, and solvent casting were employed. The effect of processing time, clay type, and clay content was systematically studied. When processed with montmorillonite-type clays, moecular weight of PHB showed decrease with respect to the loading. The release of water molecules present on the clay surfaces during processing might have reduced the molecular weight. Also, the modifier present on clay can catalyze the degradation of PHB. This was proved by different experimental techniques especially XRD studies which indicated intercalated morphology for the composites.
PHB-based materials are recently recommendded for use as biomedical devices and applicators . A very recent study to utilize composite nanofiber membrane as tissue engineering scaffold is promising. Electrospinning technology was used to prepare a composite nanofiber scaffold by mixing PHB with carboxyl multiwalled carbon nanotubes grafted PHB. The grafting of the carbon nanotubes to PHB was achieved by condensation reactions of carboxyl groups and hydroxyl groups of the components. The physical and chemical properties of the composite nanofibers were examined by various techniques. The static mechanical properties showed greater improvement than the parent polymer. The composite nanofiber membrane with large surface area, high porosity, better biodegradability, cytocompatibility and appreciable mechanical properties could be useful as a tissue engineering scaffold in the future.
Biocomposites of PHB with natural fibers is another important field of study as both are biodegradable in nature. Hosokawa et al. prepared composites of PHB with random mats of sisal and coconut fibers using compression molding technique at 180 °C by varying the fiber content between 10 and 15% . Thermal properties, water absorption, and mechanical properties of the composites were investigated before and after conditioning in a climate chamber. The composites showed appreciable thermal stability and flexural and tensile properties even after conditioning. The composites are suggested to be used as plastic bags for planting seedlings of vegetables and fruits in agriculture and disposable type of packaging trays instead of expanded polystyrene.
PHB can also be used for drug delivery applications. A series of compounds were investigated recently in this regard . The crystal growth rates of supercooled liquids were tested with the presence of a small amount of PHB. The compounds were selected according to the glass transition temperature (Tg) which was much higher than PHB (around 50 °C). They showed an acceleration in crystal growth indicating that the presence of PHB enhanced the molecular movement of the crystallizing species. However, in the compounds which have similar Tg to PHB, the polymer acted as crystal growth inhibitor. This specificity like inhibitor or accelerator to crystal growth in organic compounds is significant for applications such as drug delivery.
Drug delivery applications and biomedical devices of PHB are getting significant attention in recent years. Scaffolds, films, and membranes based on PHB are being prepared using different techniques. A highly porous structured PHB films were synthesized by Tan et al. recently . Solvent evaporation technique was used to prepare metal chloride/methanol/PHB/chloroform (MCl2/CH3OH/PHB/CHCl3) films, and the films were characterized by SEM and other techniques which confirmed the porosity. The change in concentration of metal chloride increased the average pore size. Also, they acted as nucleation sites, thereby increasing crystallinity as well as spherulitic growth.
Barouti et al. very recently reviewed the drug delivery applications of PHB in different forms . The basis of the study is that the degradation of PHB leads to the compound present in humans, 3-hydroxybutyric acid, and the PHB-based systems generally show no cytotoxicity. High hydrophobicity and crystallinity of the microbial PHB prevent its use as biomedical devices due to poor compatibility with therapeutic agents, limited encapsulation effects, and inferior drug delivery rates. The chemical modification of PHB and many synthetic routes to prepare it have increased its usage as drug delivery systems. The review detailed the different synthesis approaches, the physicochemical characteristics of the systems from nanoscale to macroscale. The comparison with PLA- and PLGA-based self-assembly systems, the details regarding the in vivo and in vitro experiments, and the steps taken for the use of PHB materials as drug delivery systems are detailed carefully. It is believed that after the systematic clinical trials based on the advances in research from past years, nanoscale PHB-based composite materials will become a valuable platform for current drug delivery systems.
Contribution of PHB-Based Materials for the Preservation of Ecosystem and Sustainability
Sustainability is defined as the study of how natural systems function, remain diverse, and produce everything it needs for the ecology to remain in balance. It also acknowledges that human civilization takes resources to sustain our modern way of life . There are various human and social factors that contribute to a healthy preservation of the sustainability. Economic development, social development, and environmental protection are considered as three pillars of sustainability. Public awareness and concern about the growing environmental pollution due to the enhanced usage of fuels and materials based on fossil origin is one of the major driving forces for the development of biopolymers. The frequent fluctuations in the petroleum prices and technological breakthrough in the field of biotechnology for the synthesis of value-added materials from low-cost natural materials are adding flavors to the development of polymers based on renewable resources. The major benefit of using bio-based polymers for various applications is their prospective contribution toward greenhouse gas balances, preservation of the ecosystem, and other positive environmental impacts over their whole life cycles.
PHB is degraded upon exposure to soil, compost, or marine sediment. The percentage of PHB polymer degrading microbes in the environment was likely to be 0.5–9.6% of the entire colonies. Most of the PHB-degrading microorganisms are isolated at ambient temperatures, and very few of them are capable of degrading PHB at a higher temperature. Biodegradation of PHB takes place in both aerobic and anaerobic atmospheres, without developing any toxic products [20, 68]. Aerobic degradation entails the transfer of PHB ultimately to CO2 and H2O. Anaerobic degradation of PHB can be evaluated by the analysis of methane emission. Many bacteria flourish in anaerobic environments and degrade PHB and its blends. PHB biodegradation is dependent on microbial activity, moisture, temperature, environment pH, molecular weight, crystallinity, etc. Copolymers having PHB monomer units are degraded rapidly than either PHB homopolymer or its copolymers. The studies showed that PHB can be degraded by 39 bacterial strains of the classes Firmicutes and Proteobacteria . During biodegradation stage, bacteria develop extracellular hydrolases that are capable of altering the polymers into the respective hydroxyl acid monomers. PHB products are hydrolysed into R-3-hydroxybutyric acid monomers, and they are metabolized by β-oxidation and the tricarboxylic acid cycle to form carbon dioxide and water under aerobic conditions .
Understanding the mechanism of degradation and analysis of the degradation products is very much essential to understand the impact of degradation of a biopolymer to its surrounding environment. Several studies were conducted to analyze the degradation profile of PHB under various conditions. Bacterial degradation of PHB in natural environments such as water soil and compost yield water and carbon dioxide . The enzymatic degradation of PHB films was reported by Kumagai et al.  at a temperature of 37 °C and pH of 7.4 using aqueous solutions of an extracellular PHB depolymerase obtained from Alcaligenes faecalis T1. It was reported that as the degree of crystallinity of PHB increased, the enzymatic degradation rate of PHB films showed a decreasing trend. The degradation started with hydrolysis of PHB chains on the surface of the film in the amorphous state and proceeded further to the crystalline phase. The same research group compared the degradation behavior of a blend of PHB with poly (ethylene oxide) (PEO) under similar conditions . It was observed that the enzymatic degradation rate of the film prepared from the blend was more rapid than that of films obtained from pure PHB. The surface morphology of the blend films after degradation revealed many holes which were due to the elution of the PEO component of the blend into the aqueous buffer. The enzymatic degradation of the PHB/PEO blend films was improved by the permeation of PHB depolymerase into the films through these holes. The fungal degradation of PHB was reported by Lee et al. . It was shown that the types of fungi present in the pond, soils, compost, sea water, hay, horse dung, etc. are capable of degrading PHB. A very interesting review on the degradation of microbial polyesters including PHB was published by Tokiwa et al. .
The degradation products of PHB can be analyzed by various analytical techniques such as HPLC, GC-MS, CZE, and NMR [76, 77, 78, 79, 80, 81, 82]. Vapor pressure analysis using HPLC as a technique to monitor degradation of PHB was reported by Polyak and co-workers . Generally, the microorganisms utilize oxygen to oxidize carbon in the polymer to carbon dioxide as one of the degradation products. Hence, consumption of oxygen and formation of carbon dioxide are good indications for the degradation of PHB. The carbon dioxide released from the degradation of PHB is used by the plants for their photosynthesis. A pictorial representation of the life cycle of PHB is shown in Fig. 3. In this manner, the life cycle of a healthy ecosystem is preserved.
The sustainability of the ecosystem can be ensured only by preserving the participating species in the ecosystem. The littering of waste plastic products can pose a serious threat to one or other components of the ecosystem, which in turn affects others and so on. The use of biopolymers including PHB helps in reducing the problems associated with the non-biodegradability of conventional petroleum-based plastics. The current world trend of development and commercialization of more and more bio-based plastics is noteworthy. In the present scenario, the contribution of PHB toward the sustainability of the ecosystem is very much valuable.
Polyhydroxybutyrate is a biopolymer which has unique properties. The sources, inherent properties, and their blends and composites were elaborately discussed in the chapter. This environment-friendly polymer has been utilized widely by various researchers to establish the degradation mechanism of a typical biopolymer. The contribution of PHB and their derivatives to the sustainability of ecosystem has been reviewed. The recent works related to PHB as a biomedical material as scaffolds, tissue engineering material, and drug delivery device were carefully analyzed. It is to be emphasized that the advancement of technology of PHB will lead it into a standout polymer in the near future itself as a wonderful commercial as well as biomedical biopolymer.
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