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Second Harmonic OCT and Combined MPM/OCT

  • Zhongping ChenEmail author
  • Shuo Tang
Reference work entry

Abstract

This chapter describes combined multiphoton microscopy (MPM) and optical coherence tomography (OCT) system and second harmonic OCT (SH-OCT) system. Second harmonic generation (SHG) enables direct imaging of optically anisotropic biological structures, such as membranes, structural proteins, microtubule ensembles, and collagen. SH-OCT combines molecular contrast of SHG with coherence gating of OCT. MPM/OCT combines molecular contrast of MPM with scattering contrast of OCT. Combining MPM and OCT onto a single platform creates a novel multimodality image technique which can acquire structural and functional imaging of tissues simultaneously. We will review the principle and technology of SH-OCT and combined MPM/OCT system, and illustrate a few examples of its applications.

Keywords

MPM Multimodal imaging Multiphoton microscopy Second harmonic Second harnomic OCT SH-OCT 

Notes

Acknowledgments

We would like to thank many of our colleagues who have contributed to the MPM/OCT and SH-OCT projects at UCI and UBC. We want to acknowledge grant support from the National Institutes of Health (R01EB-00293, R01CA-91717, R01EB-10090, R01EY-021519, R01HL-105215, P41EB-015890), Air Force Office of Scientific Research (F49620-00-1-0371), the Beckman Laser Institute Endowment, Natural Sciences and Engineering Research Council of Canada, and the Canada Foundation for Innovation.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.The Edwards Life Sciences Center for Advanced Cardiovascular TechnologyBeckman Laser InstituteIrvineUSA
  2. 2.Department of Electrical and Computer EngineeringUniversity of British ColumbiaVancouverCanada
  3. 3.Department of Biomedical Engineering, Beckman Laser InstituteUniversity of California IrvineIrvineUSA

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