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Facultative Methane Oxidizers

  • Svetlana N. DedyshEmail author
  • Peter F. Dunfield
Reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)

Abstract

Most aerobic methanotrophic bacteria grow only on C1 compounds (methane, methanol, formate, formaldehyde, or methylamines). However, facultative methanotrophs are able to use either methane or some non-C1 compounds as their sole energy sources. The existence of such bacteria was a controversial topic until facultative methanotrophy was conclusively demonstrated in members of the genus Methylocella, which are widely distributed in acidic and neutral terrestrial environments. Methylocella species are morphologically and genetically unlike obligate methanotrophs in several ways. They lack a particulate, membrane-bound methane monooxygenase that is nearly universal to methanotrophs and instead use only a soluble form of this enzyme for methane oxidation. The latter is repressed if an alternative multicarbon growth substrate is present. Methylocella spp. can grow on a range of alternative substrates including acetate, pyruvate, succinate, malate, ethanol, propane, ethane, propanol, propanediol, acetone, methyl acetate, acetol, glycerol, propionate, tetrahydrofuran, and gluconate. More limited facultative methanotrophy has also been demonstrated in several alphaproteobacterial methanotrophs of the genera Methylocystis and Methylocapsa, as well as in verrucomicrobial methanotrophs of the genus “Methylacidiphilum.” Unlike Methylocella spp., these methanotrophs all possess particulate methane monooxygenase, and growth is limited to only one or two alternative substrates (acetate, ethanol, or H2, depending on the strain). The metabolic flexibility of facultative methanotrophs offers new biotechnological potential and calls for revising our outlook on methane cycling in the environment.

Abbreviations

GAF domains

(Found in cGMP-phosphodiesterases adenylyl cyclases and FhlA, where FhlA is formate hydrogen-lyase transcriptional activator) are small-molecule-binding domains present in signal transduction proteins in organisms from all phyla

PLFA

Phospholipid fatty acid

pMMO

Particulate methane monooxygenase

RT-PCR

Reverse transcription polymerase chain reaction

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

sMMO

Soluble methane monooxygenase

TCA cycle

Tricarboxylic acid cycle

EMC pathway

Ethylmalonyl-coenzyme A pathway, PrMO Propane monooxygenase, SDIMO Soluble di-iron monooxygenase, ICM Intracytoplasmic pathway membranes

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Authors and Affiliations

  1. 1.Winogradsky Institute of Microbiology, Research Center of BiotechnologyRussian Academy of SciencesMoscowRussia
  2. 2.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

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