Encyclopedia of Signaling Molecules

Living Edition
| Editors: Sangdun Choi

Flotillin-1 (FLOT1)

Living reference work entry
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DOI: https://doi.org/10.1007/978-1-4614-6438-9_645-1
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Synonyms

Reggie-2 (Reg-2)

Historical Background

Flotillin-1 was originally identified in 1997, when Bickel et al. analyzed caveolin-rich membrane fractions from mouse lung and isolated a 45 kDa protein that was microsequenced. Degenerate primers were designed from the peptide sequences and used for PCR of mouse lung cDNA. The PCR-product was then used as a probe for screening of a 3T3-L1 adipocyte cDNA library. The characterized sequence showed similarity to the ESA protein. An antipeptide antibody identified a 47 kDa protein on Western blots. Because this protein was detected in floating, caveolin-rich, Triton X-100-insoluble membrane fractions, it was termed “flotillin-1.” The similar ESA protein (47% identity) was renamed as “flotillin-2.” Also in 1997, Schulte et al. identified and characterized two proteins that were induced in regenerating fish retinal ganglion cell axons after injury and thus were named “reggie-1” and “reggie-2.” In 1998, Lang et al. cloned the rat reggie...

Keywords

Lipid Raft Clathrin Heavy Chain Raft Association Plasma Membrane Microdomains Metalloprotease ADAM10 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

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Copyright information

© Springer Science+Business Media LLC 2016

Authors and Affiliations

  1. 1.Max F. Perutz Laboratories (MFPL)Medical University of ViennaViennaAustria