Encyclopedia of Metagenomics

Living Edition
| Editors: Karen E. Nelson

RNA in Circulation: Sources and Functions of Extracellular Exogenous RNA in the Blood

  • David GalasEmail author
  • Paul Wilmes
  • Kai Wang
Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-6418-1_105-2

Definition

RNA originated from other species.

Introduction

Molecules of many kinds are abundant in circulating blood and play a wide range of important roles, both known and unknown. These include macromolecules like proteins and nucleic acids and a wide range of smaller molecules. A number of questions are raised by recent findings of stable RNA molecules in plasma that is circulating RNA outside of the cells. Among the issues that need to be addressed are the following: What are the origins of these RNA molecules? What are the mechanisms by which they enter and are stabilized in the blood? What are their possible biological functions? And finally, what are the potential applications of these extracellular RNA molecules in diagnostic and therapeutic medicine? While the precise biological functions remain to be pinned down, extracellular RNA has been proposed as a vehicle for a previously unknown cell-cell communication system. Recent reports of the detection of foreign, exogenous...

Keywords

Lipid Vesicle Cluster Regularly Interspaced Short Palindromic Repeat Extracellular Nucleic Acid Circulate miRNA Level Model Organism Caenorhabditis Elegans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. De Guire V, Robitaille R, Tetreault N, Guerin R, Menard C, Bambace N, et al. Circulating miRNAs as sensitive and specific biomarkers for the diagnosis and monitoring of human diseases: promises and challenges. Clin Biochem. 2013;46(10–11):846–60.PubMedCrossRefGoogle Scholar
  2. Etheridge A, Gomes CP, Pereira RW, Galas D, Wang K. The complexity, function and applications of RNA in circulation. Front Genet. 2013;4:115.PubMedCentralPubMedGoogle Scholar
  3. Gerdes HH, Carvalho RN. Intercellular transfer mediated by tunneling nanotubes. Curr Opin Cell Biol. 2008;20(4):470–5.PubMedCrossRefGoogle Scholar
  4. Lawrie CH, Gal S, Dunlop HM, Pushkaran B, Liggins AP, Pulford K, et al. Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 2008;141(5):672–5.PubMedCrossRefGoogle Scholar
  5. Liu JM, Camilli A. A broadening world of bacterial small RNAs. Curr Opin Microbiol. 2010;13(1):18–23.PubMedCentralPubMedCrossRefGoogle Scholar
  6. Mandel P, Métais P. Les acides nucléiques du plasma sanguin chez l’homme. C R Acad Sci Paris. 1948;142:241–3.Google Scholar
  7. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A. 2008;105(30):10513–8.PubMedCentralPubMedCrossRefGoogle Scholar
  8. Moshiri A, Dashtbani-Roozbehani A, Najar Peerayeh S, Siadat SD. Outer membrane vesicle: a macromolecule with multifunctional activity. Human Vaccin Immunother. 2012;8(7):953–5.Google Scholar
  9. Ratajczak J, Miekus K, Kucia M, Zhang J, Reca R, Dvorak P, et al. Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. Leukemia. 2006;20(5):847–56.PubMedCrossRefGoogle Scholar
  10. Semenov DV, Baryakin DN, Brenner EV, Kurilshikov AM, Vasiliev GV, Bryzgalov LA, et al. Unbiased approach to profile the variety of small non-coding RNA of human blood plasma with massively parallel sequencing technology. Expert Opin Biol Ther. 2012;12 Suppl 1:S43–51.PubMedCrossRefGoogle Scholar
  11. Sorek R, Lawrence CM, Wiedenheft B. CRISPR-mediated adaptive immune systems in bacteria and archaea. Annu Rev Biochem. 2013;82:237–66.PubMedCrossRefGoogle Scholar
  12. Turchinovich A, Weiz L, Langheinz A, Burwinkel B. Characterization of extracellular circulating microRNA. Nucleic Acids Res. 2011;39(16):7223–33.PubMedCentralPubMedCrossRefGoogle Scholar
  13. Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007;9(6):654–9.PubMedCrossRefGoogle Scholar
  14. Villarroya-Beltri C, Gutierrez-Vazquez C, Sanchez-Cabo F, Perez-Hernandez D, Vazquez J, Martin-Cofreces N, et al. Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs. Nat Commun. 2013;4:2980.PubMedCentralPubMedCrossRefGoogle Scholar
  15. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic Acids Res. 2010;38(20):7248–59.PubMedCentralPubMedCrossRefGoogle Scholar
  16. Wang K, Li H, Yuan Y, Etheridge A, Zhou Y, Huang D, et al. The complex exogenous RNA spectra in human plasma: an interface with human gut biota? PLoS One. 2012;7(12):e51009.PubMedCentralPubMedCrossRefGoogle Scholar
  17. Wang K, Yuan Y, Li H, Cho JH, Huang D, Gray L, et al. The spectrum of circulating RNA: a window into systems toxicology. Toxicol Sci. 2013;132(2):478–92.PubMedCrossRefGoogle Scholar
  18. Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, et al. The microRNA spectrum in 12 body fluids. Clin Chem. 2010;56(11):1733–41.PubMedCrossRefGoogle Scholar
  19. Witwer KW, McAlexander MA, Queen SE, Adams RJ. Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: limited evidence for general uptake of dietary plant xenomiRs. RNA Biol. 2013;10(7):1080–6.PubMedCentralPubMedCrossRefGoogle Scholar
  20. Zhang L, Hou D, Chen X, Li D, Zhu L, Zhang Y, et al. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Res. 2012;22(1):107–26.PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Pacific Northwest Diabetes Research InstituteSeattleUSA
  2. 2.Luxembourg Centre for Systems BiomedicineUniversity of LuxembourgLuxembourgLuxembourg
  3. 3.Institute for Systems BiologySeattleUSA