Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota


  • Bertrand Bellier
  • Adrien Six
  • Véronique Thomas-Vaslin
  • David KlatzmannEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_716



A series of characteristic immunological parameters used to measure vaccine-induced immune responses, e.g.,
  1. 1.
    Innate immune responses
    1. (a)

      Dendritic cell activation (Pulendran 2004)

    2. (b)

      Inflammatory response

    3. (c)

      Complement activation

  2. 2.
    Adaptive immune responses
    1. (i)
      Antibody and B cell immune responses (Subbarao and Robertson 2006)
      • Neutralizing Ab (Ochsenbauer and Kappes 2009)

      • Non-neutralizing Ab

      • Antibody-dependent cell cytotoxicity (ADCC)

    2. (ii)
      T-cell immune responses (Seder et al. 2008)
      • Lysis (CTL)

      • Tetramer

      • Cytokines

    3. (iii)
      Lymphocyte repertoire diversity
      • Immunoscope/CDR3 spectratyping

      • TCR/Ig rearrangement quantification

      • TCR/Ig deep sequencing


See  Lymphocyte Dynamics and Repertoires, Biological Methods.

The rapid progress in flow cytometry implies that an increasing number of parameters can be looked at simultaneously, which is highly relevant for a more comprehensive assessment of...

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  1. Ochsenbauer C, Kappes JC (2009) New virologic reagents for neutralizing antibody assays. Curr Opin HIV AIDS 5:418–425Google Scholar
  2. Pulendran B (2004) Modulating vaccine responses with dendritic cells and Toll-like receptors. Immunol Rev 199:227–250PubMedGoogle Scholar
  3. Seder RA, Darrah PA, Roederer M (2008) T-cell quality in memory and protection: implications for vaccine design. Nat Rev Immunol 4:247–258Google Scholar
  4. Subbarao B, Robertson DA (2006) Assays for B lymphocyte function Chap 3, unit 3.8. In John E. Colegan et al. (eds) Current Protocols in Immunology. Wiley Inter Science, HobokenGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Bertrand Bellier
    • 1
  • Adrien Six
    • 1
  • Véronique Thomas-Vaslin
    • 1
  • David Klatzmann
    • 1
    Email author
  1. 1.Immunology-Immunopathology-ImmunotherapyUPMC University Paris 06, UMR7211, CNRS, UMR7211, INSERM, U959ParisFrance