Quantifying Lymphocyte Division, Methods
Cell division as a function of time
Active labeling of lymphocytes during division allows quantifying cell division as a function of time, as rates of accumulation, rates of proliferation, or rates of replacement of labeled cells. This correlates with DNA replication.
Dilution of a dye (e.g., CFSE) upon division allows quantifying the number of cell cycle per time units.
Decay of a genetic marker, like stable chromosome damage post radiotherapy
Cell division at a time point
DNA content of lymphocytes can be evaluated by passive staining of DNA with a fluorescent dye allowing to determine the various phases of cell cycle (see current protocols in immunology: