Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota

Quantifying Lymphocyte Division, Methods

  • Véronique Thomas-VaslinEmail author
  • Adrien Six
  • Bertrand Bellier
  • David Klatzmann
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_711



Various methodologies are available to quantify cell division (see also  Lymphocyte Labeling, Cell Division Investigation).
  1. 1.
    Cell division as a function of time
    • Active labeling of lymphocytes during division allows quantifying cell division as a function of time, as rates of accumulation, rates of proliferation, or rates of replacement of labeled cells. This correlates with DNA replication.

    • Dilution of a dye (e.g., CFSE) upon division allows quantifying the number of cell cycle per time units.

    • Decay of a genetic marker, like stable chromosome damage post radiotherapy

  2. 2.
    Cell division at a time point
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  1. Lyons AB (2000) Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Meth 243(1–2):147–154CrossRefGoogle Scholar
  2. Macallan DC, Asquith B et al (2009) Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labeled glucose. Nat Protoc 4(9):1313–1327PubMedCrossRefGoogle Scholar
  3. Thomas-Vaslin V, Altes HK et al (2008) Comprehensive assessment and mathematical modeling of T cell population dynamics and homeostasis. J Immunol 180(4):2240–2250PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Véronique Thomas-Vaslin
    • 1
    Email author
  • Adrien Six
    • 1
  • Bertrand Bellier
    • 1
  • David Klatzmann
    • 1
  1. 1.Immunology-Immunopathology-ImmunotherapyUPMC University Paris 06, UMR7211, CNRS, UMR7211, INSERM, U959ParisFrance