Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota

Lymphocyte Labeling, Cell Division Investigation

  • Véronique Thomas-VaslinEmail author
  • Adrien Six
  • Bertrand Bellier
  • David Klatzmann
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_708



Active Cell Labeling

Active cell labeling of lymphocytes during division can be used to estimate the frequency of cell division per time unit either at the level of cell or cell populations in vitro or in vivo. After in vivo incorporation of an isotope or nucleotide analogue, this method requires the sampling of cells or organs.

There is restriction usage of component according to ethics in the various species studied. Only 2H 2O and 2H-glucose can be used in vivo in humans (Fig.  1). An example of B lymphocyte dynamics investigation in humans can be found in (Macallan et al. 2005). Carbon dating by incorporation of atmospheric 14-Carbon in DNA in the 1960–1966 period is a method that can...
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  1. Asquith B, Debacq C et al (2006) Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE). Proc Biol Sci 273(1590):1165–1171PubMedCrossRefGoogle Scholar
  2. Asquith B, Borghans JA et al (2009) Lymphocyte kinetics in health and disease. Trends Immunol. 30(4):182–189PubMedCrossRefGoogle Scholar
  3. De Boer RJ, Ganusov VV et al (2006) Estimating lymphocyte division and death rates from CFSE data. Bull Math Biol 68(5):1011–1031PubMedCrossRefGoogle Scholar
  4. Macallan DC, Wallace DL et al (2005) B-cell kinetics in humans: rapid turnover of peripheral blood memory cells. Blood 105(9):3633–3640PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Véronique Thomas-Vaslin
    • 1
    Email author
  • Adrien Six
    • 1
  • Bertrand Bellier
    • 1
  • David Klatzmann
    • 1
  1. 1.Immunology-Immunopathology-ImmunotherapyUPMC University Paris 06, UMR7211, CNRS, UMR7211, INSERM, U959ParisFrance