Metabolic Flux Analysis
Metabolic flux analysis (MFA) is a popular methodology used in metabolic pathway modeling to determine fluxes in a metabolic network. It uses information about stoichiometry of the reactions, and mass balances around intracellular metabolites along with measured fluxes to obtain unmeasured fluxes (Stephanopoulos et al. 1998). Thus, MFA provides a flux map, which is a diagram showing the steady state rates (fluxes) at which the metabolic reactions are operating for a given phenotypic state.
Theory of Calculating Fluxes Using MFA
Genomic, biochemical, and strain-specific information is used to reconstruct metabolic network for sequenced organisms. Such reconstructed networks have precise information of stoichiometric relationship between reactants and products and information regarding the reversibility or irreversibility of enzymatic reactions, which are prerequisites for MFA. MFA utilizes the stoichiometric...
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