Reference Work Entry

Encyclopedia of Applied Electrochemistry

pp 522-528


Electrochemical Monitoring of Cellular Metabolism

  • Jennifer R. McKenzieAffiliated withDepartment of Chemistry, Vanderbilt University Email author 
  • , David E. CliffelAffiliated withDepartment of Chemistry, Vanderbilt University
  • , John P. WikswoAffiliated withDepartment of Physics and Astronomy, Vanderbilt University


The application of electrochemical techniques for providing insight into biological processes has become common practice over the last several decades. The study of cellular metabolism, largely ignored by the fields of molecular biology and toxicology until now [1, 2], is a vast field exploring how cellular energetics respond to internal or external influences, as well as how the metabolic state of the cell influences cellular regulation [3]. Traditionally, metabolism has been studied by following the uptake of radioactive metabolites [4, 5] or by quantifying consumption or production of analytes in flasks containing millions of cells [68]. Designing electrochemical experiments to study cellular metabolism requires extensive considerations of the cellular environment and sensor design. Many biosensors designed today are intended for point-of-care measures, where a sample of blood, urine, or cerebral spinal fluid is obtained and diluted in a buffer that allows f ...

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