The host-mediated assay tests whether the host metabolism can convert a compound (a pro-mutagen) into a mutagen, detectable by the cells passed through the host, e.g., yeast, bacterial, or animals cells injected into and then withdrawn from the abdominal cavity of a mouse previously exposed to a potential mutagen. Forward or back-mutation frequency in the cells is assessed after withdrawal from the abdominal cavity of the test animal and then plating the cells onto selective solid media or growing them in liquid media. Such a rapid assay was designed for substituting it to the slow and expensive direct mammalian assays of promutagen. (See Fig. H73); Big Blue, Muta Mouse, bioassays in genetic toxicology).
Host-mediated mutagen test (Modified after Fischer GA et al. 1974 Mutation Res. 26:501)
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(2008). Host-Mediated Assay. In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6754-9_7847
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