Cloning Vectors

Reference work entry
DOI: https://doi.org/10.1007/978-1-4020-6754-9_3171

Cloning vectors are generally plasmid, phage, or eukaryotic virus-derived linear or circular DNA capable of reproduction (most commonly in bacteria or yeast) and producing (in large numbers) molecular clones of the DNA inserted into them. Cloning vectors must have replicator mechanisms (replication drive unit) for self-propagation, multiple cloning sites (single or few recognition sites for several restriction enzymes), selectable markers (for verifying the success of molecular recombination and uncontaminated maintenance), regulatory elements for their copy number in the host (generally smaller plasmids can be present in larger number of copies), mechanisms for equal partition among the daughter cells, and genetic stability to prevent rearrangement by host enzymes. Often, it is desirable to propagate in more than one host cell (shuttle vectors). Some cloning vectors are used only for propagation of DNA, others permit expression of the genes carried, and yet others may be useful in...

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© Springer Science+Business Media 2008