Protein Interactions

Reference work entry

Interaction density (PID) is calculated as observed protein interaction/total number of possible pair-wise combinations. Conformational switches detectable by nuclear magnetic resonance relaxation experiments at the microsecond to millisecond time scale may modulate protein interactions (Koglin A et al 2006 Science 312:273). The various proteins may interact in many different ways and the ∼6000 proteins of yeast may display about 100,000 relations. In a preliminary attempt to develop a human genome-wide interaction network, ∼8100 Gateway-cloned ORFs allowed the detection of 2800 interactions (Rual J-F et al 2005 Nature [Lond] 437:1173). A new method permits prediction of interactions on the basis of protein sequence (Shen J et al 2007 Proc Natl Acad Sci USA 104:4337). Bioinformatic technology is required to separate the genuine from the spurious interactions (Jansen R et al 2003 Science 302:449). In Drosophilaa draft of 7048 proteins and 20,405 interactions were detected and at a...

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© Springer Science+Business Media 2008