Nomarski Differential Interference Contrast Microscopy

Reference work entry
DOI: https://doi.org/10.1007/978-1-4020-6754-9_11479

A phase shift is introduced artificially in unstained objects to cause a field contrast that is due to interference with diffracted light. As a result either dark field (phase contrast) or bright field contrast (Nomarski technique) can be obtained depending on the direction of the light by a quarter wave phase shift in special microscopic equipment. In colored microscopic specimens the phase shift (and contrast of the image) is brought about by the differential staining by microtechnical dyes.  resolution optical,  fluorescence microscopy,  phase-contrast microscopy,  confocal scanning,  electron microscopy

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