Cell Culture (2D and 3D) on Chip
On-Chip Cell Culture; Cell Culture on a Chip
Cell culture on chip is defined as those methods to cultivate biological living cells on two- and three-dimensional (2D and 3D) chip devices. Microchip techniques can provide many advantages for cell culture systems because the scale of the cultivated environment inside the microchip is fitted to the size of the cells. The integration of microfabrication and cell culture technologies promises great advances in basic biological research, diagnostics, biosensors, high-throughput drug screening, and so on.
Cultivating cells in vitro is one of the cornerstones of modern biology. Cell culture is an essential tool in biological science and clinical analysis, with some applications such as drug screening and biosensors. Many advances have been made in developing automated high-throughput cell culture systems. However, high cost and large size limit their application. It is therefore highly desirable to produce...
- 1.Yeon JH, Park JK (2005) Cytotoxicity test based on electrochemical impedance measurement of HepG2 cultured in microfabricated cell chip. Analyt Biochem 341:308–315 Google Scholar
- 2.Hediger S, Sayah A, Horisberger JD, et al (2001) Modular microsystem for epithelial cell culture and electrical characterization. Biosens Bioelectron 16:689–694 Google Scholar
- 3.Moriguchi H, Wakamoto Y, Sugio Y, et al (2002) An agar-microchamber cell-cultivation system: flexible change of microchamber shapes during cultivation by photo-thermal etching. Lab Chip 2:125–130 Google Scholar
- 4.El-Ali J, Sorger PK, Jensen KF (2006) Cells on chips. Nature 442:403–411 Google Scholar
- 5.Andersson H, van den Berg A (2003) Microfluidic devices for cellomics: a review. Sens Actuators B-Chem 92:315–325 Google Scholar
- 6.Balagadde FK, You LC, Hansen CL, et al (2005) Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science 309:137–140 Google Scholar
- 7.Gu W, Zhu XY, Futai N, et al (2004) Computerized microfluidic cell culture using elastomeric channels and Braille displays. Proc Natl Acad Sci USA 101:15861–15866 Google Scholar
- 8.Song JW, Gu W, Futai N, et al (2005) Computer-controlled microcirculatory support system for endothelial cell culture and shearing. Anal Chem 77:3993–3999 Google Scholar
- 9.Goto M, Sato K, Murakami A, et al (2005) Development of a microchip-based bioassay system using cultured cells. Anal Chem 77:2125–2131 Google Scholar
- 10.Chung BG, Flanagan LA, Rhee SW, et al (2005) Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip 5:401–406 Google Scholar