Gas Chromatography

  • K. A. Rittenbach
  • G. B. Baker
Reference work entry

Abstract:

Gas chromatography (GC) is commonly used for the analysis of a myriad of compounds in neurochemistry. In this chapter various aspects of GC, including inlets, columns and detectors are discussed. Appropriate sample preparation, including extraction and derivatization techniques are also covered. In the latter portion of the chapter, examples of the analysis of specific types of endogenous and exogenous compounds by GC are dealt with.

List of Abbreviations:

5‐HIAA

5‐hydroxyindole‐3‐acetic acid

5‐HT

5‐hydroxytryptamine

6‐MAM

6‐monoacetylmorphine

BDZ

benzodiazepine

DA

dopamine

ECD

electron‐capture detector

FID

Flame ionization detector

GABA

γ‐aminobutyric acid

GC

gas chromatography

GC x GC

two dimensional gas chromatography

GC‐MS

combined gas chromatography‐mass spectrometry

GHB

γ‐hydroxybutyrate

m‐, p‐HPPA

m‐, p‐hydroxyphenylacetic acid

HPLC

high performance liquid chromatography

HVA

homovanillic acid

LLE

liquid‐liquid extraction

MHPG

3‐methoxy‐4‐hydroxyphenylethylene glycol

NA

noradrenaline

NPD

nitrogen‐phosphorus detector

PEA

2‐phenylethylamine

PFBC

pentafluorobenzoyl chloride

PFBS

pentafluorobenzenesulfonyl chloride

PFPA

pentafluoropropionic anhydride

SCOT

support coated open tubular

SPE

solid phase extraction

TCA

tricyclic antidepressant

TCD

thermal conductivity detector

TMS

trimethylsilyl

WCOT

wall coated open tubular

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Notes

Acknowledgements

The authors are grateful for funding from the Canadian Institutes of Health Research (CIHR), the Canada Research Chairs program, the Davey Endowment, the Berger Endowment, and the Faculty of Graduate Studies and Research, University of Alberta.

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  • K. A. Rittenbach
  • G. B. Baker

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