Multidimensional NMR Spectroscopy
Definition
Multidimensional NMR spectroscopy (1) forms the basis of the determination of the three-dimensional structure of biomolecules (proteins, DNA, RNA) (2) by providing the resolution necessary to analyze their complex spectra. One-dimensional NMR spectra result from a Fourier transform (FT) of a directly detected time domain signal, the free induction decay (FID) that is recorded at the end of pulse sequence during the acquisition time. Higher-dimensional spectra are created by indirectly detecting further time domains. This is accomplished by systematic and independent variation of one or several delays in the pulse sequence while repeatedly acquiring an FID. In between the variable delays that result in the indirect detected dimensions are mixing sequences of varying complexity. They enable a transfer of magnetization viaseveral mechanisms resulting in correlations between signals from different nuclei. Depending on the type of interaction used to accomplish this transfer...
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