Affinity Chromatography and In Vitro Binding (Beads)
Synonyms
Affinity separation; Interaction chromatography
Definition
Affinity chromatography exploits the specific binding of a biomolecule, e.g. a protein or nucleic acid, to a ligand that is immobilised on a solid support. It is a powerful means of preparative protein isolation, but also a valuable tool for scientific discovery. The combination of affinity chromatography approaches and mass spectrometry is an efficient approach for identification and characterisation of protein complexes.
Characteristics
Affinity chromatography is a technique for isolation of biomolecules by virtue of their specific binding properties (1). The technique involves pairs of biomolecules, which specifically interact with and bind to each other, such as antibody/antigen, receptor/ligand and enzyme/substrate pairs or complementary nucleic acids (Fig. 1). Affinity chromatography is a common protein separation technique and is also used for analytical purposes and studies of protein interactions. As a...
References
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