Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine

2006 Edition
| Editors: Detlev Ganten, Klaus Ruckpaul, Walter Birchmeier, Jörg T. Epplen, Klaus Genser, Manfred Gossen, Birgit Kersten, Hans Lehrach, Hartmut Oschkinat, Patrizia Ruiz, Peter Schmieder, Erich Wanker, Christiane Nolte

Functional Assays

Reference work entry
DOI: https://doi.org/10.1007/3-540-29623-9_3700


Functional assays are encompassed within the terms high‐throughput screening and high‐content screening.


One goal of current proteomic approaches is to provide a listing of all the proteins present in either an organelle, cell type or whole tissue at a particular point in time. Although such data are extremely valuable, this is only one step towards our understanding of global protein function. Once a particular set of proteins has been identified, methodologies must be applied to screen these proteins for their cellular role. In this regard, functional assays can be defined as systematic in vivoexperiments that are designed to determine the involvement of each protein in a particular cellular pathway or biological process. This approach provides both the first clues to the cellular role of individual proteins and a means to classify the proteome into functionally related groups. In keeping with this definition, the term functional assays is not used here to...

This is a preview of subscription content, log in to check access.


  1. 1.
    Strausberg RL, Feingold EA, Grouse LH et al (2002) Generation and initial analysis of more than 15,000 full‐length human and mouse cDNA sequences. Proc Nat Acad Sci USA99:16899–16903PubMedCrossRefGoogle Scholar
  2. 2.
    Cesareni G, Panni S, Nardelli G et al (2002) Can we infer peptide recognition specificity mediated by SH3 domains? FEBS Lett. 513:38–44PubMedCrossRefGoogle Scholar
  3. 3.
    Huh WK, Falvo JV, Gerke LC et al (2003) Global analysis of protein localization in budding yeast. Nature 425:686–691PubMedCrossRefGoogle Scholar
  4. 4.
    Simpson JC, Wellenreuther R, Poustka A et al (2000) Systematic subcellular localisation of novel proteins identified by large‐scale cDNA sequencing. EMBO Rep 1:287–292PubMedCrossRefGoogle Scholar
  5. 5.
    Liebel U, Starkuviene V, Erfle H et al (2003) A microscope‐based screening platform for large scale functional analysis in intact cells. FEBS Lett 554:394–398PubMedCrossRefGoogle Scholar
  6. 6.
    Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature 411:107–110PubMedCrossRefGoogle Scholar
  7. 7.
    Aridor M, Hannan LA (2002) Traffic jams II: an update of diseases on intracellular transport. Traffic 3:781–790PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Cell Biology and Biophysics Programme, European Molecular Biology Laboratory (EMBL)HeidelbergGermany