Synonyms
Functional assays are encompassed within the terms high‐throughput screening and high‐content screening.
Definition
One goal of current proteomic approaches is to provide a listing of all the proteins present in either an organelle, cell type or whole tissue at a particular point in time. Although such data are extremely valuable, this is only one step towards our understanding of global protein function. Once a particular set of proteins has been identified, methodologies must be applied to screen these proteins for their cellular role. In this regard, functional assays can be defined as systematic in vivoexperiments that are designed to determine the involvement of each protein in a particular cellular pathway or biological process. This approach provides both the first clues to the cellular role of individual proteins and a means to classify the proteome into functionally related groups. In keeping with this definition, the term functional assays is not used here to...
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Strausberg RL, Feingold EA, Grouse LH et al (2002) Generation and initial analysis of more than 15,000 full‐length human and mouse cDNA sequences. Proc Nat Acad Sci USA99:16899–16903
Cesareni G, Panni S, Nardelli G et al (2002) Can we infer peptide recognition specificity mediated by SH3 domains? FEBS Lett. 513:38–44
Huh WK, Falvo JV, Gerke LC et al (2003) Global analysis of protein localization in budding yeast. Nature 425:686–691
Simpson JC, Wellenreuther R, Poustka A et al (2000) Systematic subcellular localisation of novel proteins identified by large‐scale cDNA sequencing. EMBO Rep 1:287–292
Liebel U, Starkuviene V, Erfle H et al (2003) A microscope‐based screening platform for large scale functional analysis in intact cells. FEBS Lett 554:394–398
Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature 411:107–110
Aridor M, Hannan LA (2002) Traffic jams II: an update of diseases on intracellular transport. Traffic 3:781–790
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2005 Springer-Verlag
About this entry
Cite this entry
Simpson, J.C. (2005). Functional Assays. In: Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-29623-9_3700
Download citation
DOI: https://doi.org/10.1007/3-540-29623-9_3700
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-44244-8
Online ISBN: 978-3-540-29623-2
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences