Reference Work Entry

Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine

pp 1506-1509

Protein Interaction Analysis: Chemical Cross‐Linking

  • Owen W. NadeauAffiliated withDepartment of Biochemistry and Molecular Biology, Kansas University Medical Center, Kansas City Email author 


Protein conjugation; bioconjugation; protein chemical modification; covalent coupling


Protein interaction analysis is one of the main fields of functional proteomics, covering an array of traditional protein chemical and newly developing techniques that center primarily either on identifying components or determining mechanisms of protein‐protein interactions in both short‐lived and long‐lived protein complexes. The stability of a complex is determined, in part, by the surface contact area or protein interface formed between interacting proteins. Surface contact areas are generally unique for interacting pairs of proteins, with some proteins containing several protein‐binding domains that permit multiple contacts in large protein complexes. Chemical cross‐linking, an extension of protein chemical modification, is an established technique used to join two or more of the proteins in a complex covalently using multi‐functional chemical compounds, termed

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