Historical Background
The short isoform of Sorcin (soluble resistance-related calcium-binding protein) (a 19,000-dalton peptide) was isolated for the first time by Meyers and coworkers in vincristine-resistant Chinese hamster cells (DC-3FVCRd-5). A similar protein (Mr = 19,000; pI = 5.7) was also found in a vincristine-resistant mouse line (Meyers and Biedler 1981). The gene of Sorcin (SRI) codifying for the longest isoform was identified around the multidrug resistance gene region of human chromosome 7q21.1.
The gene can generate at least four alternative transcripts, i.e., isoforms A (a primary transcript of 15 kb divided in eight exons and seven introns, transcribed in a 198-amino-acid-long protein), B, C, and D, yielding shorter forms of the protein, lacking part of the N-terminal domain and/or of the final residues of the C-terminal domain. The isoform A was identified by Van...
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References
Colotti G, Zamparelli C, Verzili D, Mella M, Loughrey CM, Smith GL, Chiancone E. The W105G and W99G Sorcin mutants demonstrate the role of the D helix in the Ca(2+)-dependent interaction with annexin VII and the cardiac ryanodine receptor. Biochemistry. 2006;45(41):12519–29.
Colotti G, Poser E, Fiorillo A, Genovese I, Chiarini V, Ilari A. Sorcin, a calcium binding protein involved in the multidrug resistance mechanisms in cancer cells. Molecules. 2014;19:13976–89.
Fowler MR, Colotti G, Chiancone E, Higuchi Y, Seidler T, et al. Complex modulation of L-type Ca(2+) current inactivation by Sorcin in isolated rabbit cardiomyocytes. Pflugers Arch. 2009;457:1049–60.
Franceschini S, Ilari A, Verzili D, Zamparelli C, Antaramian A, et al. Molecular basis for the impaired function of the natural F112L Sorcin mutant: x-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor. FASEB J. 2008;22:295–306.
Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E. The crystal structure of the sorcin calcium binding domain provides a model of Ca2+−dependent processes in the full-length protein. J Mol Biol. 2002;317(3):447–58.
Ilari A, Fiorillo A, Poser E, Lalioti VS, Sundell GN, Ivarsson Y, Genovese I, Colotti G. Structural basis of Sorcin-mediated calcium-dependent signal transduction. Sci Rep. 2015;5:16828.
Lalioti VS, Ilari A, O’Connell DJ, Poser E, Sandoval IV, Colotti G. Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis. PLoS One. 2014;9(1):e85438.
Lokuta AJ, Meyers MB, Sander PR, Fishman GI, Valdivia HH. Modulation of cardiac ryanodine receptors by Sorcin. J Biol Chem. 1997;272(40):25333–8.
Maddalena F, Laudiero G, Piscazzi A, Secondo A, Scorziello A, Lombardi V, Matassa DS, Fersini A, Neri V, Esposito F, Landriscina M. Sorcin induces a drug-resistant phenotype in human colorectal cancer by modulating Ca(2+) homeostasis. Cancer Res. 2011;71:7659–69.
Maki M, Kitaura Y, Satoh H, Ohkouchi S, Shibata H. Structures, functions and molecular evolution of the penta-EF-hand Ca2+−binding proteins. Biochim Biophys Acta. 2002;1600(1–2):51–60.
Matsumoto T, Hisamatsu Y, Ohkusa T, Inoue N, Sato T, et al. Sorcin interacts with sarcoplasmic reticulum Ca(2+)-ATPase and modulates excitation contraction coupling in the heart. Basic Res Cardiol. 2005;100:3250–62.
Mella M, Colotti G, Zamparelli C, Verzili D, Ilari A, Chiancone E. Information transfer in the penta-EF-hand protein Sorcin does not operate via the canonical structural/functional pairing. A study with site-specific mutants. J Biol Chem. 2003;278(27):24921–8.
Meyers MB, Biedler JL. Increased synthesis of a low molecular weight protein in vincristine-resistant cells. Biochem Biophys Res Commun. 1981;99(1):228–35.
Meyers MB, Zamparelli C, Verzili D, Dicker AP, Blanck TJ, Chiancone E. Calcium-dependent translocation of sorcin to membranes: functional relevance in contractile tissue. FEBS Lett. 1995;357(3):230–4.
Noordeen NA, Meur G, Rutter GA, Leclerc I. Glucose-induced nuclear shuttling of ChREBP is mediated by sorcin and Ca(2+) ions in pancreatic β-cells. Diabetes. 2012;61:574–85.
Tong W, Sun D, Wang Q, Suo J. Sorcin enhances metastasis and promotes epithelial-to-mesenchymal transition of colorectal cancer. Cell Biochem Biophys. 2015;72(2):453–9.
Van der Bliek AM, Meyers MB, Biedler JL, Hes E, Borst P. A 22-kd protein (Sorcin/V19) encoded by an amplified gene in multidrug-resistant cells, is homologous to the calcium-binding light chain of calpain. EMBO J. 1986;5(12):3201–8.
Van der Bliek AM, Baas F, Van der Velde-Koerts T, Biedler JL, Meyers MB, Ozols RF, et al. Genes amplified and overexpressed in human multidrug-resistant cell lines. Cancer Res. 1988;48(21):5927–32.
Xie X, Dwyer MD, Swenson L, Parker MH, Botfield MC. Crystal structure of calcium-free human Sorcin: a member of the penta-EF-hand protein family. Protein Sci. 2001;10(12):2419–25.
Zamparelli C, Macquaide N, Colotti G, Verzili D, Seidler T, Smith GL, Chiancone E. Activation of the cardiac Na(+)-Ca(2+) exchanger by Sorcin via the interaction of the respective Ca(2+)-binding domains. J Mol Cell Cardiol. 2010;49(1):132–41.
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Colotti, G., Ilari, A. (2018). Sorcin. In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, Cham. https://doi.org/10.1007/978-3-319-67199-4_101842
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DOI: https://doi.org/10.1007/978-3-319-67199-4_101842
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