Definition
Metagenomics refers to the use of DNA sequencing to determine the phylogenetic and functional gene complement of a sample, such as microbial community DNA in soil. A shotgun metagenomic approach relies on sequencing of total DNA extracted from a given sample, without prior cloning into a vector.
Soil as a Microbial Habitat
Soil has several unique properties compared to other microbial habitats that are important to consider when discussing the topic of soil metagenomics. Currently, there are few published soil metagenome studies, although there is a large interest in the scientific community on this topic based on attendance at workshops and conferences and funding of the TerraGenome NSF-sponsored Research Coordination Network (www.terragenome.org). Although metagenomics is revealing new information about phylogenetic and functional genes in some soils, it is not possible to adopt the information available to date to all soils.
There are different classes of soils that vary...
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References
Artursson V, Jansson JK. Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. Appl Environ Microbiol. 2003;69:6208–15.
Artursson V, Finlay RD, Jansson JK. Combined bromodeoxyuridine immunocapture and terminal restriction fragment length polymorphism analysis highlights differences in the active soil bacterial metagenome due to Glomus mosseae inoculation of plant species. Environ Microbiol. 2005;7:1952–6.
Chen Y, Murrell JC. When metagenomics meets stable-isotope probing: progress and perspectives. Trends Microbiol. 2010;18:157–63.
Chourey K, Jansson JK, VerBerkmoes, et al. Direct cellular lysis/protein extraction protocol for soil metaproteomics. J Proteome Res. 2010;9:6615–22.
Delmont TO, Robe, Cecillon S, et al. Accessing the soil metagenome for studies of microbial diversity. Appl Environ Microbiol. 2011;77:1315–24.
Delmont TO, Prestat E, Keegan KP, et al. Structure, fluctuation and magnitude of a natural grassland soil metagenome. ISMEJ. 2012;6:1677–87.
Dumont MG, Radejewski SM, Miguez CB, et al. Identification of a complete methane monooxygenase operon from soil by combining stable isotope probing and metagenomics analysis. Environ Microbiol. 2006;8:1240–50.
Ekkers DM, Silvia Cretoiu M, Kielak AM, et al. The great screen anomaly- a new frontier in product discovery through functional metagenomics. Appl Microbiol Biotechnol. 2012;93:1005–20.
Fierer N, Bradford MA, Jackson RB. Toward and ecological classification of soil bacteria. Ecology. 2007;88:1354–64.
Gilbert JA, Meyer F, Jansson JK, et al. The Earth Microbiome Project: meeting report of the 1st EMP meeting on sample selection and acquisition. Stand Gen Sci. 2010;3:3.
Hjort K, Bergström M, Adesina M, et al. Chitinase genes revealed and compared in bacterial isolates, DNA extracts and a metagenomic library from a phytopathogen suppressive soil. FEMS Microbiol Ecol. 2010;71:197–207.
Holben WE. GC fractionation allows comparative total microbial community analysis, enhances diversity assessment, and facilitates detection of minority populations of minority populations of bacteria. In: Bruijn FJ, editor. Handbook of molecular microbial ecology I: metagenomics and complementary approaches. Hoboken: Wiley; 2010. p. 183–96.
Holben WE, Jansson JK, Chelm BK, et al. DNA probes method for the detection of specific microorganisms in the soil bacterial community. Appl Environ Microbiol. 1988;54:703–11.
Mackelprang R, Waldrop MP, DeAngelis KM, et al. Deep metagenome sequencing illuminates permafrost response to thaw. Nature. 2011;480:368–71.
Maraha N, Backman A, Jansson JK. Monitoring physiological status of GFP-tagged Pseudomonas fluorescens SBW25 under different nutrient conditions and in soil by flow cytometry. FEMS Microbiol Ecol. 2004;51:123–32.
Pell J, Hintze, Canino-Koning R, et al. A single pass approach to reducing sampling variation, removing errors and scaling de novo assembly of shotgun sequences. Proc Natl Acad Sci U S A. 2012. doi:10.1073/pnas.1121464109.
Rousk J, Bååth E, Brookes PC, et al. Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME J. 2010;4:1340–51.
Schloss PD, Handelsman J. Toward a census of bacteria in soil. PLoS Comp Biol. 2006;2(7):e92.
Sjoling S, Stafford W, Cowan DA. Soil metagenomics: exploring and exploiting the soil microbial gene pool. In: van Elsas JD, Jansson JK, Trevors JT, editors. Modern soil microbiology. Boca Raton: CRC Press/Taylor & Francis Group; 2007. p. 409–30.
Steven B, Gallegos-Graves LV, Starkenburg SR, et al. Targeted and shotgun metagenomic approaches provide different descriptions of dryland soil microbial communities in a manipulated field study. Environ Microbiol Rep. 2012;4:248–56.
Tringe SG, von Mering C, Kobayashi A, et al. Comparative metagenomics of microbial communities. Science. 2005;308:554–7.
Van Elsas JD, Jansson JK, Sjoling S, et al. The soil metagenome–a recalcitrant, yet explorable goldmine for gene hunters. Trends Biotechnol. 2008;26:591–691.
Yergeau E, Hogues H, Whyte LG, et al. The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses. ISMEJ. 2010;4:1206–14.
Yergeau E, Sanschagrin S, Beaumier D, et al. Metagenomic analysis of the bioremediation of diesel-contaminated high arctic soils. PLoS One. 2012;7(1):e30058.
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Jansson, J. (2013). Soil Metagenomics. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_701-4
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DOI: https://doi.org/10.1007/978-1-4614-6418-1_701-4
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