Bioprocessing of Plant In Vitro Systems

1. Front Matter

   Pages i-xvii

   PDF

2. Historical Background

   1. Front Matter

      Pages 1-1

      PDF

   2. History of Plant Biotechnology Development

      • Ivelin Pantchev, Goritsa Rakleova, Atanas Pavlov, Atanas Atanassov

      Pages 3-37

   3. Plant Tissue Culture Technology: Present and Future Development

      • Svetla Yancheva, Violeta Kondakova

      Pages 39-63

3. Metabolic Phytochemistry

   1. Front Matter

      Pages 65-65

      PDF

   2. Metabolite Profiling of In Vitro Plant Systems

      • Strahil Berkov, Liliya Georgieva, Borjana Sidjimova, Milena Nikolova

      Pages 67-83

   3. Microbial Transformations of Plant Secondary Metabolites

      • Blaga Mutafova, Pedro Fernandes, Sava Mutafov, Strahil Berkov, Atanas Pavlov

      Pages 85-124

4. Secondary Metabolites

   1. Front Matter

      Pages 125-125

      PDF

   2. Engineering Cell and Organ Cultures from Medicinal and Aromatic Plants Toward Commercial Production of Bioactive Metabolites

      • Krasimir Rusanov, Atanas Atanassov, Ivan Atanassov

      Pages 127-160

   3. Agrobacterium rhizogenes-Mediated Transformation of Plants for Improvement of Yields of Secondary Metabolites

      • Tatiana V. Matveeva, Sophie V. Sokornova

      Pages 161-202

   4. Amaryllidaceae Alkaloid Accumulation by Plant In Vitro Systems

      • Dominique Laurain-Mattar, Agata Ptak

      Pages 203-223

   5. Sustainable Production of Polyphenols and Antioxidants by Plant In Vitro Cultures

      • Iryna Smetanska

      Pages 225-269

   6. Production of Iridoid and Phenylethanoid Glycosides by In Vitro Systems of Plants from the Buddlejaceae, Orobanchaceae, and Scrophulariaceae Families

      • Ewelina Piątczak, Renata Grąbkowska, Halina Wysokińska

      Pages 271-293

   7. Taxus Cell Cultures: An Effective Biotechnological Tool to Enhance and Gain New Biosynthetic Insights into Taxane Production

      • Heriberto Vidal-Limon, Raúl Sanchez-Muñoz, Abbas Khojasteh, Elisabeth Moyano, Rosa M. Cusido, Javier Palazon

      Pages 295-316

   8. Challenges for the Cultivation of Plant Cells on the Example of Hypericum perforatum and Taxus chinensis

      • Mariam Gaid, Thomas Wucherpfennig, Stephan Scholl, Ludger Beerhues, Rainer Krull

      Pages 317-341

   9. Mass Production of Artemisinin Using Hairy Root Cultivation of Artemisia annua in Bioreactor

      • Nivedita Patra, Ashok K. Srivastava

      Pages 343-359

   10. Plant In Vitro Systems as Sources of Food Ingredients and Additives

       • Radka Vrancheva, Nadezhda Petkova, Ivan Ivanov

       Pages 361-392

   11. Safety Assessment and Regulations for Food Ingredients Derived from Plant In Vitro Systems

       • Angel Angelov, Velitchka Gotcheva

       Pages 393-409

5. Bioreactor Technology and Monitoring

   1. Front Matter

      Pages 411-411

      PDF

   2. Bioreactor Technology for Sustainable Production of Plant Cell-Derived Products

      • Sören Werner, Rüdiger W. Maschke, Dieter Eibl, Regine Eibl

      Pages 413-432

This handbook presents how plant in vitro technologies can overcome current limitations in the production of important plant-derived substances. It explains the advantages of plant in vitro technologies, notably the independence from climatic and soil conditions and the ability to synthesize diverse bioactive substances under controlled conditions. Apart from making diverse metabolites, which can be used e.g. as pharmaceuticals, agrochemicals, flavors, colors, biopesticides or food additives, more easily and more efficiently available, the methods described in this handbook also offer the advantage that rare and threatened plants, which provide access to interesting and desired substances, can be better protected, when the substances are harvested from suitable plant in vitro systems. In times of increasing demand for natural plant-derived products, the described methodologies will be key to ensuring efficient and sustainable access to plant-derived products. They will also help and support in the research and investigation of plant secondary metabolites.


Despite these advantages, still only few substances are being produced at industrial scale by in vitro plant cell cultivation systems to date. This handbook therefore advertises the recent achievements and research in the field, focused on solving limitations in yield and bioprocessing conditions. Leading experts summarize the methodology, which can help overcome drawbacks like low yields of target products or problems associated with the cultivation in bioreactors. Readers will find comprehensive information on fundamentals for using different types of plants in vitro as matrix for sustainable production of valuable secondary metabolites. The handbook summarizes the core information on phytochemistry, bioreactor technology and monitoring of plant cells and tissues in bioprocesses. It also discusses selected applications and safety assessment of food and cosmetic ingredients from plant cell and tissue.

• Plant cells
• Plant biotechnology
• Plant tissue
• Plant secondary metabolites biotransformation
• Bioreactor cultivation
• Process monitoring and optimization
• Plant organ cultures
• Production of bioactive compounds
• Hairy roots cultures
• Plant cell cultivation
• Secondary metabolites yield improvement
• Bioreactor technology
• Medicinal and aromatic plants
• Metabolomics

• University of Food Technologies, Plovdiv, Bulgaria

  Atanas Pavlov

• Bioprocess Engineering, Institute of Food Technology and Bioprocess Engineering, TU Dresden, Dresden, Germany

  Thomas Bley

Prof. Dr. Atanas Pavlov is Professor for Technology of bioactive substances at the University of Food Technologies, Plovdiv, Bulgaria, and Professor and Head of laboratory of the Laboratory of Applied Biotechnologies at The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences. He has received his MSc in Biotechnology from the University of Food Technologies, Plovdiv, Bulgaria in 1992 and a PhD in Biotechnology from The Stephan Angeloff institute of Microbiology, Bulgarian Academy of Sciences in 1998. Prof. Pavlov worked as post-doctoral researcher at the Laboratoire de Biologie et Physiologie Végétales, University of Reims Champagne (France) and at the Institute of Food Technology and Bioprocess Engineering, TU Dresden, Germany (with support by a Marie Curie Host Fellowship). Afterwards he returned to Bulgaria to become Associate Professor in the Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences. Since 2010 he is full professor and heading the laboratory, additionally holding a professorship at Plovdiv University of Food Technologies.
Prof. Pavlov’s scientific interests include plant biotechnologies, bioprocess engineering, plant secondary metabolites-isolation, purification, and elucidation, and food biocativities. He has been and is co-editor, advisory board member and reviewer for numerous high impact scientific journals in the fields of biotechnology, microbiology, biochemistry, food chemistry and plant sciences.


Prof. Dr. Thomas Bley is the Chair of Bioprocess Engineering at the Dresden University of Technology (Germany). He studied mathematics in Dresden, graduating with a Diploma in 1975. After that he became a scientist at the Institute of Biotechnology (IBT) at the Academy of Science of the GDR in Leipzig, and received his PhD from the Academy in 1981. In 1990 Prof. Bley completed a habilitation in biotechnology at the University of Leipzig and subsequently became the head of the division Biosignals at the IBT. From 1994-1996 he was the Head of the division Biotechnology at the Faculty of Biosciences, Pharmacy and Psychology at the University of Leipzig, Germany, before returning to Dresden.
Prof. Bley is elected full member of the German and the Saxonian Academies of Sciences and editor-in-chief of the journal “Engineering in Life Sciences”. His research focuses on biomonitoring and biosignals, modeling and controlling of bioprocesses, biocatalysis in heterogeneous systems, white biotechnology and plant cells in bioreactors.