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Pyrosequencing® Protocols pp 1–13Cite as

The History of Pyrosequencing®

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 373)

Abstract

One late afternoon in the beginning of January 1986, bicycling from the lab over the hill to the small village of Fullbourn, the idea for an alternative DNA sequencing technique came to my mind. The basic concept was to follow the activity of DNA polymerase during nucleotide incorporation into a DNA strand by analyzing the pyrophosphate released during the process. Today, the technique is used in multidisciplinary fields in academic, clinical, and industrial settings all over the word. The technique can be used for both single-base sequencing and whole-genome sequencing, depending on the format used.

Keywords

  • Magnetic Bead
  • Nucleotide Incorporation
  • Pyrosequencing Method
  • Apyrase Activity
  • Medical Research Council Laboratory

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, AlbaNova University Center, Stockholm, Sweden

    Pål Nyrén

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  1. Pål Nyrén
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Editor information

Editors and Affiliations

  1. Division of Oncology, Washington University School of Medicine, St. Louis, MO

    Sharon Marsh

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© 2007 Humana Press Inc.

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Nyrén, P. (2007). The History of Pyrosequencing®. In: Walker, J.M., Marsh, S. (eds) Pyrosequencing® Protocols. Methods in Molecular Biology™, vol 373. Humana Press. https://doi.org/10.1385/1-59745-377-3:1

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  • DOI: https://doi.org/10.1385/1-59745-377-3:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-645-0

  • Online ISBN: 978-1-59745-377-6

  • eBook Packages: Springer Protocols

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