Abstract
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
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Moorhead, G.B.G., Haystead, T.A.J., MacKintosh, C. (2007). Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose. In: Moorhead, G. (eds) Protein Phosphatase Protocols. Methods in Molecular Biology, vol 365. Springer, Totowa, NJ. https://doi.org/10.1385/1-59745-267-X:39
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DOI: https://doi.org/10.1385/1-59745-267-X:39
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-1-58829-711-2
Online ISBN: 978-1-59745-267-0
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